- AF6-120.1 (See other available formats)
- Regulatory Status
- Other Names
- MHC class II
- Mouse (BALB/c) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- C57BL/10J splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).
Does not react with other haplotypes (e.g., d, f, q, r, s).
(PubMed link indicates BioLegend citation)
- Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
- Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
- Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
- Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
- Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
- Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
- Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
- Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
- Product Citations
AB_10613291 (BioLegend Cat. No. 116421)
AB_10613473 (BioLegend Cat. No. 116422)
- MHC class II
B cell and activated T cells, APCs of H-2b mice
- Antigen presentation
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
- Gene ID
- 14961 View all products for this Gene ID
- View information about I-Ab on UniProt.org
|Biotin anti-mouse I-Ab||AF6-120.1||FC|
|FITC anti-mouse I-Ab||AF6-120.1||FC|
|PE anti-mouse I-Ab||AF6-120.1||FC|
|Purified anti-mouse I-Ab||AF6-120.1||FC, IHC-F, IP|
|Alexa Fluor® 488 anti-mouse I-Ab||AF6-120.1||FC|
|Alexa Fluor® 647 anti-mouse I-Ab||AF6-120.1||FC|
|PerCP/Cyanine5.5 anti-mouse I-Ab||AF6-120.1||FC|
|APC anti-mouse I-Ab||AF6-120.1||FC|
|PE/Cyanine7 anti-mouse I-Ab||AF6-120.1||FC|
|Pacific Blue™ anti-mouse I-Ab||AF6-120.1||FC|
|APC/Fire™ 750 anti-mouse I-Ab||AF6-120.1||FC|
|APC/Cyanine7 anti-mouse I-Ab||AF6-120.1||FC|
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-mouse I-Ab
FITC anti-mouse I-Ab
PE anti-mouse I-Ab
Purified anti-mouse I-Ab
Alexa Fluor® 488 anti-mouse I-Ab
Alexa Fluor® 647 anti-mouse I-Ab
PerCP/Cyanine5.5 anti-mouse I-Ab
APC anti-mouse I-Ab
PE/Cyanine7 anti-mouse I-Ab
Pacific Blue™ anti-mouse I-Ab
APC/Fire™ 750 anti-mouse I-Ab
APC/Cyanine7 anti-mouse I-Ab