PE anti-human CD9 Antibody

Pricing & Availability
Clone
HI9a (See other available formats)
Workshop
V P018
Other Names
Tetraspanin, MRP-1, DRAP-24
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HI9a_PE_060807
Human peripheral blood platelets stained with HI9a PE
  • HI9a_PE_060807
    Human peripheral blood platelets stained with HI9a PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
312105 25 tests $100
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312106 100 tests $220
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Description

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cattle (Bovine, Cow), Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Rhesus, Sheep (Ovine)
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Valentijn A, et al. 2013. Hum Reprod. 28:2695. PubMed
  2. Huygens C, et al. 2015. J Biol Chem. 290: 20105-20116. PubMed
  3. Etzerodt A, et al. 2017. Sci Rep. 7:40286. PubMed
Publication Library
RRID
AB_2075893 (BioLegend Cat. No. 312105)
AB_2075892 (BioLegend Cat. No. 312106)

Antigen Details

Structure
Tetraspan family, type III transmembrane protein, 24 kD
Distribution

Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells

Function
Adhesion, platelet activation, B cell development
Ligand Receptor
Associates with CD63, CD81, CD82 and CD36, binds PSG17
Cell Type
Platelets, B cells, Lymphocytes, Granulocytes, Endothelial cells, Epithelial cells, Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Miao WM, et al. 2001 Blood 97:1689.
2. Ellerman DA, et al. 2003 Mol. Biol Cell. (Epub ahead of print).
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

Gene ID
928 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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