- Other Names
- Mouse CD4 T Cell Negative Selection, Mouse CD4 T Cell Untouched, Mouse CD4 T Cell Depletion, Mouse CD4 T Cell Enrichment, Th1, Th2, Th9, Th17, Th22, T Follicular Helper
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- Product Citations
- publications
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A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with CD4 (clone RM4-4) PE and CD3ε (145-2C11) APC. -
Non CD4+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
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For Cat# 480005:
- 100 µl of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD45R/B220, CD49b, CD105, I-A/I-E (MHC II), TER-119/Erythroid, TCR-γδ
- 100 µl Streptavidin Nanobeads
For Cat# 480006:
- 1 ml of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD45R/B220, CD49b, CD105, I-A/I-E (MHC II), TER-119/Erythroid, TCR-γδ
- 1 ml Streptavidin Nanobeads
For Cat# 480033:
- 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD45R/B220, CD49b, CD105, I-A/I-E (MHC II), TER-119/Erythroid, TCR-γδ
- 2 vials of 1 ml Streptavidin Nanobeads each
Product Details
- Reactivity
- Mouse
- Formulation
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Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
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The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer. - Application Notes
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This kit is designed for the isolation of untouched CD4+ T cells from lymphoid tissues.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 9X -
Application References
(PubMed link indicates BioLegend citation) -
- Dong L, et al. 2016. Sci. Rep. 6:36598. (PubMed)
- Product Citations
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
