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MojoSort™ Mouse CD4 T Cell Isolation Kit

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MCD4TCellIsoKit_1_051215
A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with CD4 (clone RM4-4) PE and CD3ε (145-2C11) APC.
  • MCD4TCellIsoKit_1_051215
    A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with CD4 (clone RM4-4) PE and CD3ε (145-2C11) APC.
  • MCD4TCellIsoKit_2_051215
Cat # Size Price Quantity Check Availability Save
480005 10 tests $113
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480006 100 tests $483
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480033 200 tests $654
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Description

Non CD4+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480005:

  • 100 µl Biotin-Antibody Cocktail
  • 100 µl Streptavidin Nanobeads

For Cat# 480006:

  • 1 ml Biotin-Antibody Cocktail
  • 1 ml Streptavidin Nanobeads

For Cat# 480033:

  • 2 vials of 1 ml Biotin-Antibody Cocktail each
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Verified Reactivity
Mouse
Formulation
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched CD4+ T cells from lymphoid tissues.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 9X

Application References

(PubMed link indicates BioLegend citation)
  1. Dong L, et al. 2016. Sci. Rep. 6:36598. (PubMed)
Product Citations
  1. Fernandez-Perez R, et al. 2021. Front Immunol. 12:687443. PubMed
  2. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  3. Pei S, et al. 2021. J Exp Med. 218:. PubMed
  4. Lin CH, et al. 2021. J Exp Med. 218:. PubMed
  5. Ecker M, et al. 2022. Elife. 11:. PubMed
  6. Guzman–Genuino RM, et al. 2020. Front Immunol. 2.429861111. PubMed
  7. Dong L, et al. 2017. Sci Rep. 10.1038/srep36598. PubMed
  8. Elyada E, et al. 2019. Cancer Discov. 1.140277778. PubMed
  9. Pan YG, et al. 2017. Front Immunol. 1.322222222. PubMed
  10. Tatsumi N, et al. 2021. Sci Immunol. 6:eabg0336. PubMed
  11. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  12. Bai C, et al. 2020. Mol Ther Oncolytics. 17:9. PubMed
  13. Tang X, et al. 2022. Cell Rep. 41:111673. PubMed
  14. Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed
  15. Bocchini C, et al. 2016. Blood. 128(26):3061-3072. PubMed
  16. Woodring T, et al. 2022. iScience. 25:104934. PubMed
  17. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  18. Feng P, et al. 2021. Theranostics. 11:9503. PubMed
  19. Kumar V, et al. 2022. JACC Basic Transl Sci. 7:1038. PubMed
  20. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  21. Nomura S, et al. 2019. Sci Rep. 9:17335. PubMed
  22. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  23. Qin J, et al. 2020. Cancer Immunol Res. 8:1426. PubMed

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD4 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 4    Revision Date: 01/26/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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