Glial Cell Marker Antibody Sampler Kit

Pricing & Availability
Other Names
Please refer to individual product datasheets.
Ave. Rating
Submit a Review
Product Citations
publications
Glial_Cell_Marker_Antbody_Sampler_kit_1_060618
IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_1_060618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_2_060618
    IHC staining of anti-CX3CR1 antibody (clone 8E10.D9) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with clone 8E10.D9 at 20 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection following by hematoxylin and bluing solution counterstain.
  • Glial_Cell_Marker_Antbody_Sampler_kit_3_060618
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_4_060618
    IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_5_060618
    Western blot of purified anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Glial_Cell_Marker_Antbody_Sampler_kit_6_060618
    Western blot of anti-Myelin Basic Protein antibody (clone P82H9) and isotype-matched IgG1 control. Blots were incubated with anti-MBP at 1 µg/ml (left) or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20 µg; rat brain lysates: 20 µg.
Cat # Size Price Quantity Avail. Save
899904 1 kit $380
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The Glial Cell Marker Antibody Sampler Kit offers flexibility for sampling and detection of specific cell types in the central nervous system.  Antibodies included in this kit target specific markers that are used for assessment of protein expression or identification of microglia, astrocytes and oligodendrocytes.

Product Details
Technical data sheet

Kit Contents

Kit Contents
Specificity Format Clone Size Reactivity Isotype
Anti-P2RY12 Purified S16007D 25 μg Mouse Rat IgG2b, κ
Anti-CX3CR1 Purified 8E10.D9 25 μg Human Mouse IgG1, λ
Anti-GFAP Purified SMI 24 25 μg Human, Mouse, Rat Mouse IgG2b, κ
Anti-Myelin CNPase Purified SMI 91 25 μg Human, Rat, Mouse Mouse IgG1, κ
Anti-Myelin Basic Protein Purified P82H9 25 μg Human, Rat Mouse IgG1, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

IHC-P, WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by immunohistochemical staining on formalin-fixed paraffin-embedded tissue or Western blotting. For immunohistochemistry, the suggested uses of these reagents are as follows:

Anti-P2RY12: 5.0 - 10 µg/ml
Anti-CX3CR1: 10 - 20 µg/ml
Anti-GFAP: 1.0 - 5.0 µg/ml
Anti-Myelin CNPase: 0.1 - 1.0 µg/ml

For Western blotting, the suggested uses of these reagents are as follows:

Anti-Myelin Basic Protein: 0.1 - 1.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

RRID
AB_2734654 (BioLegend Cat. No. 899904)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/08/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account