Purified anti-P2RY12 Antibody

Pricing & Availability
Clone
S16007D (See other available formats)
Other Names
P2Y12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled, 12
Isotype
Rat IgG2b, κ
Ave. Rating
1 reviews
Product Citations
publications
S16007D_PURE_P2RY12_Antibody_2_032618
IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • S16007D_PURE_P2RY12_Antibody_2_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • S16007D_PURE_P2RY12_Antibody_3_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • S16007D_PURE_P2RY12_Antibody_4_032618
    Single cell suspension of mouse brain tissue was stained with purified anti-P2RY12 antibody (clone S16007D, blue histogram) or rat IgG isotype control (red histogram), followed by anti-rat antibody conjugated to APC for detection.
  • S16007D_PURE_P2RY12_Antibody_5_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on PFA-fixed frozen mouse brain tissue. The tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5ug/ml of Alexa Fluor® 594 conjugated Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. The image was captured using a 40X objective.
  • S16007D_PURE_P2RY12_Antibody_6_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on 4%-formaldehyde fixed mouse brain tissue. Following thick (100µm) vibratome sectioning, the slices were blocked for 1h at RT with 10%NGS and 1%BSA in PBST (pH=7.4 PBS + 0.1% Tween20), incubated with the primary antibody at 5 µg/ml overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 2h at RT. CX3CR1-GFP signal enabled validation of the microglial-specific anti-P2RY12 staining (pseudocolored red). The above images are maximal intensity projections from 40µm-thick z-stacks captured with a 60X objective on a confocal laser scanning microscope. The images are a courtesy of Dr. Alberto Sepulveda-Rodriguez and Dr. Stefano Vicini, Department of Pharmacology and Physiology, Interdisciplinary Program in Neuroscience, Georgetown University Medical Center.
Cat # Size Price Quantity Avail. Save
848001 25 µg $95
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848002 100 µg $250
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Description

Purinergic Receptor P2Y (P2RY12) is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. P2RY12 is not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. P2RY12 is a highly selective marker for microglia that specifically distinguishes these cells from other myeloid cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse P2RY12.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For flow cytometric staining. a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research.

Learn more about the validation testing we performed on clone S16007D by watching our Scientific Poster Video.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed
Publication Library
RRID
AB_2650633 (BioLegend Cat. No. 848001)
AB_2650634 (BioLegend Cat. No. 848002)

Antigen Details

Structure
P2RY12 is a 347 amino acid protein with a molecular mass of 39.8 kD.
Distribution

Tissue distribution: platelets, brain (microglia), lung, appendix, pituitary and adrenal glands.
Cellular distribution: cell membrane and cytosol.

Function
G-protein coupled receptor that inhibits the adenylyl cyclase second messenger system.
Ligand Receptor
ADP and ATP.
Cell Type
Microglia, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Purinergic Receptors
Antigen References

1. Orr AG, et al. 2009. Nat. Neurosci. 12(7):872-8.

Gene ID
70839 View all products for this Gene ID
UniProt
View information about P2RY12 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 07/09/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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