FluoroFix™ Buffer

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Other Names
Fixative, Paraformaldehyde
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Product Citations
Cat # Size Price Quantity Avail. Save
422101 200 tests $130
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FluoroFix™ Buffer is a ready-to-use buffer, specially formulated for fixation of immunofluorescence stained cells, optimized to stabilize tandem dyes. It can be used as the final resuspension of the cell pellet in immunofluorescence staining procedures. FluoroFix™ Buffer is provided as 100 mL.This quantity is sufficient for 200 tests.

Product Details
Technical data sheet

Product Details

Storage & Handling
The buffer solution should be stored between 2°C and 8°C.

FC, ICFC - Quality tested
ICC - Reported in the literature, not verified in house

Recommended Usage

After final wash in the staining procedures (please refer to Cell Surface Immunofluorescence Staining Protocol), discard supernatant and resuspend the cell pellet in 0.5 ml/tube FluoroFix™ Buffer and analyzed with flow cytometry. If the samples can not be analyzed within 4 hours, resuspend the cell pellet in 0.5 ml/tube FluoroFix™ Buffer and incubate in the dark for 30 minutes at room temperature. Wash 1X with Biolegend Cell Staining Buffer (cat# 420201) and resuspend in 0.5ml/tube Cell Staining Buffer, cover the tubes.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application References

(PubMed link indicates BioLegend citation)
  1. Lewis ND, et al. 2014. J Neuroimmunol. 277:26. PubMed
  2. Shen L, et al. 2015. Cancer Immunol Res. 3:136. PubMed
Product Citations
  1. Lewis N, et al. 2014. J Neuroimmunol. 277:26. PubMed
  2. Shen L, et al. 2015. Cancer Immunol Res. 3:136. PubMed
  3. Kwek S, et al. 2015. Cancer Immunol Res. 3: 1008-1016. PubMed
  4. Wright A, et al. 2016. J Immunol. 196: 4877 - 4884. PubMed
  5. Surdziel E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183679. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Kang YJ, et al. 2007. Nature Immunol. 8:601.

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Related FAQs

Can I keep my stained cells in your FluoroFix™ Buffer (422101) for many days before acquisition?

Keeping stained cells in any fixative for longer periods of time is not recommended. You can use Fluorofix™ buffer as recommended on the data sheet, followed by washing with cell staining buffer and then at the last step, resuspend your fixed cells in cell staining buffer till you acquire your samples in a day or two.

Go To Top Version: 3    Revision Date: 07/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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