FITC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
3 reviews
Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
300405 25 tests $20
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300452 100 µg $45
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300406 100 tests $45
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300440 500 tests $185
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
Product Citations
  1. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  2. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  3. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  4. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  5. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  6. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  7. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  8. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  9. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  10. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  11. Whitfield S, et al. 2017. J Immunol. 10.4049/jimmunol.1601525. PubMed
  12. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  13. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  14. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  15. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  16. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  17. Yamada E 2018. Cell host & microbe. 23:110. PubMed
  18. Barrow AD, et al. 2018. Cell. 172:534. PubMed
Publication Library
RRID
AB_314059 (BioLegend Cat. No. 300405)
AB_2564148 (BioLegend Cat. No. 300452)
AB_314060 (BioLegend Cat. No. 300406)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand Receptor
Peptide antigen bound to MHC
Cell Type
T cells, NKT cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
915 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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