FITC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
300405 25 tests $22
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300452 100 µg $50
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300406 100 tests $50
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300440 500 tests $204
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  2. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  3. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  4. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  5. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  6. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  7. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  8. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  9. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  10. Chen L, et al. 2018. Immunology. 155:123. PubMed
  11. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  12. Coufal S, et al. 2019. Cells. 8. PubMed
  13. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  14. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  15. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  16. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  17. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  18. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  19. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  20. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  21. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  22. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  23. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
  24. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  25. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  26. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  27. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  28. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  29. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  30. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  31. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  32. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  33. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  34. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  35. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  36. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  37. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  38. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  39. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  40. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  41. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  42. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  43. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  44. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  45. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  46. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  47. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  48. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  49. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  50. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  51. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  52. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
RRID
AB_314059 (BioLegend Cat. No. 300405)
AB_2564148 (BioLegend Cat. No. 300452)
AB_314060 (BioLegend Cat. No. 300406)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC, CyTOF®, IHC-F, IP, Activ, WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC, ICC, IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC, ICC, IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC, ICC, IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC, CyTOF®, IHC-F, IP, Activ, WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC, CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC, FC, SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD3

  • Biotin anti-human CD3

  • FITC anti-human CD3

  • PE anti-human CD3

  • PE/Cyanine5 anti-human CD3

  • Purified anti-human CD3

  • Alexa Fluor® 647 anti-human CD3

  • Alexa Fluor® 488 anti-human CD3

  • Pacific Blue™ anti-human CD3

  • PE/Cyanine7 anti-human CD3

  • Alexa Fluor® 700 anti-human CD3

  • APC/Cyanine7 anti-human CD3

  • PerCP anti-human CD3

  • PerCP/Cyanine5.5 anti-human CD3

  • Brilliant Violet 421™ anti-human CD3

  • Brilliant Violet 570™ anti-human CD3

  • Ultra-LEAF™ Purified anti-human CD3

  • Purified anti-human CD3 (Maxpar® Ready)

  • Alexa Fluor® 594 anti-human CD3

  • PE/Dazzle™ 594 anti-human CD3

  • Brilliant Violet 510™ anti-human CD3

  • Brilliant Violet 605™ anti-human CD3

  • Brilliant Violet 711™ anti-human CD3

  • Brilliant Violet 650™ anti-human CD3

  • APC/Fire™ 750 anti-human CD3

  • Pacific Blue™ anti-human CD3

  • Brilliant Violet 785™ anti-human CD3

  • PE/Dazzle™ 594 anti-human CD3

  • TotalSeq™-A0034 anti-human CD3

  • TotalSeq™-B0034 anti-human CD3

  • TotalSeq™-C0034 anti-human CD3

  • PE anti-human CD3

  • PE/Cyanine7 anti-human CD3

  • KIRAVIA Blue 520™ anti-human CD3

  • Spark Violet™ 538 anti-human CD3 Antibody

  • TotalSeq™-D0034 anti-human CD3

  • Spark Blue™ 574 anti-human CD3 Antibody

  • GMP Pacific Blue™ anti-human CD3

  • GMP PE anti-human CD3

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