FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
6 reviews
Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
109805 50 µg $45
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109806 500 µg $105
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Burman A, et al. 2007. Blood. 110:1064. PubMed
  2. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  3. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  4. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  5. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  6. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  7. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  8. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  9. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  10. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  11. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  12. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  13. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  14. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  15. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  16. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  17. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  18. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  19. Hou Y 2018. Immunity. 49:490. PubMed
  20. Kleppe M 2018. Cancer cell. 33:29. PubMed
  21. Edgar LJ 2018. Cell chemical biology. 26(1):131. PubMed
  22. Malik A 2018. Immunity. 49:515. PubMed
  23. Sade-Feldman M 2018. Cell. 175:998. PubMed
  24. Wu B 2018. Immunity. 49:886. PubMed
  25. Zhu YP 2018. Cell reports. 24:2329. PubMed
  26. Stewart I 2018. Immunity. 49:477. PubMed
  27. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  28. Uchil PD, et al. 2019. Cell Host Microbe. 25:87. PubMed
  29. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  30. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  31. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
Publication Library
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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