APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
109813 25 µg $30
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109814 100 µg $105
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  2. Markey K, et al. 2012. Blood. 119:5918. PubMed
  3. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  4. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  5. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  6. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  7. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  8. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  9. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  10. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  11. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  12. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  13. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  14. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  15. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  16. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  17. Hirota K 2018. Immunity. 48:1220. PubMed
  18. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  19. Xu X 2018. Cell. 175:1336. PubMed
  20. Burrack KS 2018. Immunity. 48:760. PubMed
  21. Kleppe M 2018. Cancer cell. 33:29. PubMed
  22. Habtetsion T 2018. Cell metabolism. 28:228. PubMed
  23. Lu Y 2018. Cancer cell. 33:1048. PubMed
  24. Cohen SB 2018. Cell host & microbe. 24:439. PubMed
  25. Firl DJ 2018. eLife. 7: e33051. PubMed
  26. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  27. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  28. Hayatsu N, et al. 2017. Immunity. 47:268. PubMed
  29. Goldstein JM, et al. 2019. Cell Rep. 27:1254. PubMed
Publication Library
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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