Alexa Fluor® 700 anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
104_Alx700_020608
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 700
  • 104_Alx700_020608
    C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
109821 25 µg $85
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109822 100 µg $195
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Kang S, et al. 2011. J Exp Med. 208:747. PubMed
  2. Semmrich M, et al. 2011. Mucosal Immunol. 0.3125. PubMed
  3. Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed
  4. Basu S, et al. 2013. J Immunol Methods. 396:163. PubMed
  5. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  6. DeFranco D 2014. Proc Natl Acad Sci U S A. 111:3224. PubMed
  7. Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed
  8. Kretschmer B, et al. 2015. Immunobiology. 220: 964-975. PubMed
  9. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  10. XS R, et al. 2015. Diabetes. 64 90. PubMed
  11. Amos J, et al. 2015. J Virol. 89: 9485-9498. PubMed
  12. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
  13. Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
  14. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  15. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  16. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  17. Zaid A,et al. 2017. J Immunol. 10.4049/jimmunol.1700571. PubMed
  18. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  19. Trompette A 2018. Immunity. 48:992. PubMed
  20. Kleppe M 2018. Cancer cell. 33:29. PubMed
  21. Vasamsetti SB 2018. Immunity. 49:93. PubMed
  22. Booth CAG 2018. Cancer cell. 33:274. PubMed
  23. Adams NM 2018. Immunity. 48:1172. PubMed
  24. Geary CD 2018. Cell reports. 24:1949. PubMed
  25. Cohen SB 2018. Cell host & microbe. 24:439. PubMed
  26. Turner JS, et al. 2018. Cell Rep. 25:1395. PubMed
  27. Bradley CP, et al. 2017. Cell Host Microbe. 22:697. PubMed
  28. Hayatsu N, et al. 2017. Immunity. 47:268. PubMed
  29. Wu J, et al. 2017. Immunity. 47:1114. PubMed
  30. Goldstein JM, et al. 2019. Cell Rep. 27:1254. PubMed
  31. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  32. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  33. Page N, et al. 2018. Immunity. 48:937. PubMed
  34. Louveau A, et al. 2018. Nat Neurosci. 21:1380. PubMed
Publication Library
RRID
AB_493730 (BioLegend Cat. No. 109821)
AB_493731 (BioLegend Cat. No. 109822)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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