D2-40 Lymphatic Endothelial Marker Monoclonal Antibody

Pricing & Availability
Clone
D2-40 (See other available formats)
Isotype
Mouse IgG1
D2-40_Purified_D2-40_Lymphatic_Endothelial_Marker_Antibody_IHC_030615
Immunohistochemistry showing lymphatic channels with staining of the endothelium. The adjacent small blood vessels show no staining of their endothelium. The section is from muscularis propria of bowel (40X).
  • D2-40_Purified_D2-40_Lymphatic_Endothelial_Marker_Antibody_IHC_030615
    Immunohistochemistry showing lymphatic channels with staining of the endothelium. The adjacent small blood vessels show no staining of their endothelium. The section is from muscularis propria of bowel (40X).
Cat # Size Price Quantity Avail. Save
916604 6 mL $285
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916601 100 µL $308
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916603 6 mL $336
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916602 1 mL $558
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Description

This antibody is effective in immunohistochemistry (IHC).

Clone D2-40 reacts with a O-linked sialoglycoprotein (MW 40 kD) found on lymphatic endothelium, fetal testis and on the surface of testicular germ cell tumors. Clone D2-40 (D240) has shown staining in lymphatic channel endothelium but not in the adjacent capillary.

Concentrated Format:
Cat. No. 916601 (Formerly: SIG-3730-100) [0.1 ml]
Cat. No. 916602 (Formerly: SIG-3730-1000) [1 ml]

Prediluted Formats: 
Cat. No. 916603 (Formerly: SIG-3730-16) [6 ml]-ready to use for automated stainers
Cat. No. 916604 (Formerly: SIG-3730-26) [6 ml]-ready to use for manual staining

Both are ready-to-use with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.

Product Details
Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody D2-40 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of D2-40 Lymphatic Endothelial Marker. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity
Human
Immunogen
This antibody was developed using M2A.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
Ammonium sulfate precipitated TCS.
Storage & Handling
Store between 2°C and 8°C.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: Not required
Incubation: 60 minutes at room temperature

Application References

(PubMed link indicates BioLegend citation)
  1. Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC) PubMed
  2. Choi WW, et al. 2005. Mod Pathol. 18:143. PubMed
  3. Chu AY, et al. 2005. Mod Pathol. 18:105.
  4. Dumoff KL, et al. 2005. Mod Pathol. 18:97.
  5. Galambos C, Nodit L. 2005. Ped Dev Pathol. 8:181.
  6. Fogt F, et al. 2004. Oncol Rep. 11:47.
  7. Fogt F, et al. 2004. Int J Molec Med. 13:681.
Product Citations
  1. Hamanaka T, et al. 2011. Invest Ophthalmol Vis Sci. 52:8849-8861. PubMed
  2. Choi W, et al. 2005. Mod Pathol. 18:143-152. PubMed
  3. Bordry N, et al. 2018. Oncoimmunology. 7:e1462878. PubMed
  4. Belle M et al. 2017. Cell. 169(1):161-173 . PubMed
  5. Dunk C, et al. 2019. Front Endocrinol (Lausanne). 0.527777778. PubMed
  6. Shen CN, et al. 2019. EBioMedicine. 47:98. PubMed
RRID
AB_2565185 (BioLegend Cat. No. 916604)
AB_2565182 (BioLegend Cat. No. 916601)
AB_2565184 (BioLegend Cat. No. 916603)
AB_2565183 (BioLegend Cat. No. 916602)
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 06/10/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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