D2-40 Lymphatic Endothelial Marker Monoclonal Antibody

Pricing & Availability
Clone
D2-40 (See other available formats)
Other Names
Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Previously
Signet Catalog# 730-001
Signet Catalog# 730-01
Signet Catalog# 730-16 L1 Predilute
Signet Catalog# 730-26 L2 Predilute
Covance Catalog# SIG-3730
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
D2-40_Purified_D2-40_Lymphatic_Endothelial_Marker_Antibody_IHC_030615
Immunohistochemistry showing lymphatic channels with staining of the endothelium. The adjacent small blood vessels show no staining of their endothelium. The section is from muscularis propria of bowel (40X).
  • D2-40_Purified_D2-40_Lymphatic_Endothelial_Marker_Antibody_IHC_030615
    Immunohistochemistry showing lymphatic channels with staining of the endothelium. The adjacent small blood vessels show no staining of their endothelium. The section is from muscularis propria of bowel (40X).
Cat # Size Price Quantity Avail. Save
916604 6 ml $285
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916601 100 µl $308
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916603 6 ml $336
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916602 1 ml $558
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody D2-40 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of D2-40 Lymphatic Endothelial Marker. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using M2A.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
Ammonium sulfate precipitated TCS.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: Not required
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Clone D2-40 reacts with a O-linked sialoglycoprotein (MW 40 kD) found on lymphatic endothelium, fetal testis and on the surface of testicular germ cell tumors. Clone D2-40 (D240) has shown staining in lymphatic channel endothelium but not in the adjacent capillary.

Concentrated Format:
Cat. No. 916601 (Formerly: SIG-3730-100) [0.1 ml]
Cat. No. 916602 (Formerly: SIG-3730-1000) [1 ml]

Prediluted Formats:
Cat. No. 916603 (Formerly: SIG-3730-16) [6 ml]
Cat. No. 916604 (Formerly: SIG-3730-26) [6 ml]

Both are ready-to-use with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.

Application References

(PubMed link indicates BioLegend citation)
  1. Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC) PubMed
  2. Choi WW, et al. 2005. Mod Pathol. 18:143. PubMed
  3. Chu AY, et al. 2005. Mod Pathol. 18:105.
  4. Dumoff KL, et al. 2005. Mod Pathol. 18:97.
  5. Galambos C, Nodit L. 2005. Ped Dev Pathol. 8:181.
  6. Fogt F, et al. 2004. Oncol Rep. 11:47.
  7. Fogt F, et al. 2004. Int J Molec Med. 13:681.
Product Citations
  1. Hamanaka T, et al. 2011. Invest Ophthalmol Vis Sci. 52:8849-8861. PubMed
  2. Choi W, et al. 2005. Mod Pathol. 18:143-152. PubMed
  3. Bordry N, et al. 2018. Oncoimmunology. 7:e1462878. PubMed
Publication Library
RRID
AB_2565185 (BioLegend Cat. No. 916604)
AB_2565182 (BioLegend Cat. No. 916601)
AB_2565184 (BioLegend Cat. No. 916603)
AB_2565183 (BioLegend Cat. No. 916602)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Cancer Biomarkers
Gene ID
10630 View all products for this Gene ID
UniProt
View information about D2-40 Lymphatic Endothelial Marker on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

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Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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