Purified anti-Podoplanin (Lymphatic Endothelial Marker) Antibody

Pricing & Availability
Clone
D2-40 (See other available formats)
Other Names
Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_D2-40_PURE_Podoplanin_Antibody_3_IHC_012618
IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker) antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1.3 µg/ml of the primary antibody at room temperature for 60 minutes. BioLegend's Ultra-Streptavidin (USA) HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_D2-40_PURE_Podoplanin_Antibody_3_IHC_012618
    IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker) antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1.3 µg/ml of the primary antibody at room temperature for 60 minutes. BioLegend's Ultra-Streptavidin (USA) HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_D2-40_PURE_Podoplanin_Antibody_2_IHC_060215
    IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker)antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 3_D2-40_PURE_Podoplanin_Antibody_1_WB_111417
    Western blot analysis of cell lysates from HeLa using Podoplanin Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
Cat # Size Price Quantity Avail. Save
916605 25 µg $105
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916606 100 µg $225
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Description

Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
M2A protein derived from human germ cell tumors.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated
ELISA Detection, ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA Detection5 and immunocytochemistry4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC-P)
  2. Choi WW, et al. 2005. Mod. Pathol. 18:143. (IHC-P)
  3. Chu AY, et al. 2005. Mod. Pathol. 18:105. (IHC-P) PubMed
  4. Yoon C, et al. 2008. Blood 112:1129. (ICC) PubMed
  5. Schacht V, et al. 2005. American Journal of Pathology 166:913. (ELISA Detection, WB) PubMed
  6. Marks A, et al. 1999. British Journal of Cancer 80:569.
Product Citations
  1. Yoon C, et al. 2008. Blood. 112:1129-1138. PubMed
  2. Schacht V, et al. 2005. Am J Pathol. 166:913-921. PubMed
  3. Chu A, et al. 2005. Mod Pathol. 18:105-110. PubMed
Publication Library
RRID
AB_2565819 (BioLegend Cat. No. 916605)
AB_2565820 (BioLegend Cat. No. 916606)

Antigen Details

Structure
35 kD.
Distribution

Membrane and plasma membrane.

Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction
Cytoskeletal signaling.
Biology Area
Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 05/03/2018

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