APC/Fire™ 750 anti-mouse H-2Kd/H-2Dd Antibody

Pricing & Availability
Clone
34-1-2S (See other available formats)
Regulatory Status
RUO
Other Names
MHC Class I
Isotype
Mouse (C3H) IgG2a, κ
Ave. Rating
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Product Citations
publications
A.
34-1-2S_APC-FIRE-750_H2K-H-2D_His-FC_122923
C57BL/6 mouse splenocytes were stained with anti-mouse H-2Kd/H-2Dd (clone 34-1-2S) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • A.
34-1-2S_APC-FIRE-750_H2K-H-2D_His-FC_122923
    C57BL/6 mouse splenocytes were stained with anti-mouse H-2Kd/H-2Dd (clone 34-1-2S) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • B.
34-1-2S_APC-FIRE-750_H2K-H-2D_Dot-2-FC_122923
    C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (clone GK1.5) PE and anti-mouse H-2Kd/H-2Dd (clone 34-1-2S) APC/Fire™ 750 (left) or mouse IgG2a, κ APC/Fire™ 750 isotype control (right).
Compare all formats See APC/Fire™ 750 spectral data
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114719 25 µg $106
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114720 100 µg $270
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Description

The 34-1-2S antibody reacts with the H-2Kd/H-2Dd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd/H-2Dd haplotype. H-2Kd/H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BDF Splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. Sester M, et al. 2000. J. Biol. Chem. 34:113.
RRID
AB_3097451 (BioLegend Cat. No. 114719)
AB_3097451 (BioLegend Cat. No. 114720)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd/H-2Dd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
  3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Kd/H-2Dd on UniProt.org
Go To Top Version: 1    Revision Date: 12/29/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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