Alexa Fluor® 647 anti-mouse H-2Kd/H-2Dd Antibody

Pricing & Availability
Clone
34-1-2S (See other available formats)
Other Names
MHC Class I
Isotype
Mouse (C3H) IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
34-1-2S_Alx647_122607
C57BL/6 mouse splenocytes stained with 34-1-2S Alexa Fluor® 647
  • 34-1-2S_Alx647_122607
    C57BL/6 mouse splenocytes stained with 34-1-2S Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
114712 100 µg $195
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Description

The 34-1-2S antibody reacts with the H-2Kd/H-2Dd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd/H-2Dd haplotype. H-2Kd/H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BDF Splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. Sester M, et al. 2000. J. Biol. Chem. 34:113.
Product Citations
  1. Wu J, et al. 2009. Clin Cancer Res. 1.063888889. PubMed
  2. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
Publication Library
RRID
AB_493063 (BioLegend Cat. No. 114712)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd/H-2Dd haplotype

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
  3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Kd/H-2Dd on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 04/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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