FITC anti-mouse H-2Kd/H-2Dd Antibody anti-H-2Ld, H-2Db

Pricing & Availability

Clone: 34-1-2S (See other available formats)
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ
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Product Citations: publications

34-1-2s — C57BL/6 mouse splenocytes stained with 34-1-2S PE

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114706

500 µg

$293

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Description

The 34-1-2S antibody reacts with the H-2K^d/H-2D^d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K^d/H-2D^d haplotype. H-2K^d/H-2D^d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

BDF Splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation², complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.

Application References (PubMed link indicates BioLegend citation)

Ozato K, et al. 1982. Transplantation 34:113.
Sester M, et al. 2000. J. Biol. Chem. 34:113.

Product Citations

Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
Li X, et al. 2022. Nat Commun. 13:2794. PubMed
Liu X, et al. 2020. Nature. . PubMed
Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed
Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed

RRID

AB_313605 (BioLegend Cat. No. 114706)

Antigen Details

Structure

MHC class I

Distribution

Nucleated cells of the H-2K^d/H-2D^d haplotype

Function

Antigen presentation

Ligand/Receptor

CD3/TCR, CD8

Biology Area

Immunology, Innate Immunity

Molecular Family

MHC Antigens

Antigen References

Watts C. 1997. Ann. Rev. Immunol. 15:821.
Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
York IA, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID

14964 View all products for this Gene ID 14972 View all products for this Gene ID

UniProt

View information about H-2Kd/H-2Dd on UniProt.org

Documentation

Reviews

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Other Formats

View All H-2Kd/H-2Dd Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Alexa Fluor® 647 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PerCP/Cyanine5.5 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE/Cyanine7 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC/Fire™ 750 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Spark Violet™ 423 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 04/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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