APC anti-mouse CD120a (TNF R Type I/p55) Antibody

Pricing & Availability
Clone
55R-286 (See other available formats)
Other Names
TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype
Armenian Hamster IgG
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Product Citations
publications
55R-286_APC_120109
Mouse fibroblast NIH/3T3 cells stained with 55R-286 APC
  • 55R-286_APC_120109
    Mouse fibroblast NIH/3T3 cells stained with 55R-286 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
113005 25 µg $105
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113006 100 µg $305
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Description

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Pinckard JK, et al. 1997. J. Biol Chem. 272:10784. (IP)
  2. Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)
  3. Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)
  4. Bell JH, et al. 2007. J. Leukocyte Biol. 82:173. (FC)
  5. Kuroishi T, et al. 2008. J. Leukocyte Biol. 83:912.
  6. de Oliveria DC, et al. 2014. Cytokine. 69:218. PubMed
  7. Kumari S, et al. 2014. Elife. 3:3422. PubMed
Product Citations
  1. Oliveira D, et al. 2014. Cytokine. 69:218. PubMed
  2. Kumari S, et al. 2014. Elife. 3:3422. PubMed
  3. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  4. Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed
Publication Library
RRID
AB_2208780 (BioLegend Cat. No. 113005)
AB_2208779 (BioLegend Cat. No. 113006)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution
Variety of cell types at low levels
Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.

Gene ID
21937 View all products for this Gene ID
UniProt
View information about CD120a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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