APC anti-human/mouse/rat CD278 (ICOS) Antibody

Pricing & Availability
C398.4A (See other available formats)
Regulatory Status
Other Names
Inducible COStimulatory molecule, H4
Armenian Hamster IgG
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Product Citations
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A APC
  • C398dot4A_APC_091907
    PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A APC
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313509 25 µg $119
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313510 100 µg $237
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ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type
Host Species
Armenian Hamster
Mouse T cell clone D10.G4.1
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that reagents be titrated for optimal performance in the particular application.

Excitation Laser
Red Laser (633 nm)
Application Notes
  1. The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
Application References

(PubMed link indicates BioLegend citation)
  1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
  2. Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
  3. Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
  4. Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed
  2. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  3. Karim F, et al. 2021. Elife. 10:. PubMed
  4. Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
  5. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  6. Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
  7. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
  8. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  9. Cassotta A, et al. 2020. J Exp Med. 217:00:00. PubMed
  10. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  11. Zhang SL, et al. 2022. Gut Microbes. 14:2046246. PubMed
  12. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  13. Bonnet B, et al. 2016. J Immunol. 197: 188 - 198. PubMed
  14. NULL, et al. 2022. Cell. 185:916. PubMed
  15. Llibre A, et al. 2016. J Immunol. 196: 2085 - 2094. PubMed
  16. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  17. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  18. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  19. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  20. Lim S, et al. 2017. Sci Rep. 7:40040. PubMed
  21. Deakin A, et al. 2014. PLoS One. 9:107490. PubMed
  22. Cassotta A, et al. 2020. Eur J Immunol. . PubMed
AB_416333 (BioLegend Cat. No. 313509)
AB_416334 (BioLegend Cat. No. 313510)

Antigen Details

CD28/CTLA-4, 47-57 kD

Activated T cells, subset of thymocytes

Costimulates T cell activation, proliferation, humoral immune response
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95.

Gene ID
100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
View information about CD278 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD278 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human/mouse/rat CD278 (ICOS) C398.4A FC,IP,Costim,SB
Biotin anti-human/mouse/rat CD278 (ICOS) C398.4A FC
FITC anti-human/mouse/rat CD278 (ICOS) C398.4A FC
PE anti-human/mouse/rat CD278 (ICOS) C398.4A FC
APC anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Alexa Fluor® 488 anti-human/mouse/rat CD278 (ICOS) C398.4A FC,SB
Alexa Fluor® 647 anti-human/mouse/rat CD278 (ICOS) C398.4A FC,IHC-F
PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Pacific Blue™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Brilliant Violet 421™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Brilliant Violet 510™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
APC/Cyanine7 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
PE/Dazzle™ 594 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Alexa Fluor® 700 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
APC/Fire™ 750 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Brilliant Violet 785™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Brilliant Violet 605™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Ultra-LEAF™ Purified anti-human/mouse/rat CD278 (ICOS) C398.4A FC,IP,Costim
GoInVivo™ Purified anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Brilliant Violet 650™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
TotalSeq™-B0171 anti-human/mouse/rat CD278 (ICOS) C398.4A PG
TotalSeq™-C0171 anti-human/mouse/rat CD278 (ICOS) C398.4A PG
TotalSeq™-A0171 anti-human/mouse/rat CD278 (ICOS) C398.4A PG
Brilliant Violet 750™ anti-human/mouse/rat CD278 (ICOS) C398.4A FC
TotalSeq™-D0171 anti-human/mouse/rat CD278 (ICOS) Antibody C398.4A PG
PE/Cyanine5 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS) C398.4A FC
Go To Top Version: 2    Revision Date: 02/07/2013

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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