FITC anti-human/mouse/rat CD278 (ICOS) Antibody

Pricing & Availability
Clone
C398.4A (See other available formats)
Other Names
Inducible COStimulatory molecule, H4
Isotype
Armenian Hamster IgG
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Product Citations
publications
C398_4A_FITC_072007
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A FITC
  • C398_4A_FITC_072007
    PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
313505 25 µg $65
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313506 100 µg $140
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Description

ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Mouse, Rat, Rhesus, Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T cell clone D10.G4.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and in vitro costimulation of T cell activation1,3,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 313512).

Application References

(PubMed link indicates BioLegend citation)
  1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
  2. Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
  3. Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
  4. Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
Product Citations
  1. Rankin A, et al. 2011. J Immunol. 186:667. PubMed
  2. Tonti E, et al. 2012. J Immunol. 188:3217. PubMed
  3. Colineau L, et al. 2015. PLoS One. 10: e0140978. PubMed
  4. Bolton D, et al. 2017. PLoS Pathogens. 13(6):e1006445. PubMed
Publication Library
RRID
AB_416329 (BioLegend Cat. No. 313505)
AB_416330 (BioLegend Cat. No. 313506)

Antigen Details

Structure
CD28/CTLA-4, 47-57 kD
Distribution

Activated T cells, subset of thymocytes

Function
Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor
B7h/B7RP-1/GL-50
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95.

Gene ID
100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
UniProt
View information about CD278 (ICOS) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/07/2013

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