- GK1.5 (See other available formats)
- Other Names
- L3T4, T4
- Rat IgG2b, κ
- Ave. Rating
- 2 reviews
- Product Citations
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CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.Product Details
- Antibody Type
- Host Species
- Mouse CTL clone V4
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry of frozen tissue sections, a concentration range of 2.5-5 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, and immunoprecipitation1,2. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
- Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
- Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
- Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
- Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
- Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
- Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
- Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
- Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
- Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
- Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
- Product Citations
AB_493519 (BioLegend Cat. No. 100426)
AB_389324 (BioLegend Cat. No. 100424)
- Ig superfamily, 55 kD
Majority of thymocytes, T cell subset
- TCR co-receptor, T cell activation
- MHC class II molecule
- Cell Type
- Dendritic cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
- Gene ID
- 12504 View all products for this Gene ID
- View information about CD4 on UniProt.org
- I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
- TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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