- SA376A4 (See other available formats)
- Other Names
- My10, gp105-120, mucosialin
- Rat IgG2a, λ
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- Product Citations
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CD34, also known as gp105-120, is a highly glycosylated type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD, with two generated by alternative splicing. CD34 is expressed on hematopoietic progenitors, endothelial cells, brain, and testis. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands, and mediates the attachment of stem cells to the extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.Product Details
- Antibody Type
- Host Species
- Mouse CD34 transfected cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies.
AB_2629649 (BioLegend Cat. No. 152205)
- Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD.
Hematopoietic progenitors, endothelial cells, brain and testis tissues.
- Cell adhesion and homing.
- L-selectin and E-selectin.
- Cell Type
- Endothelial cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood. 79:2288.
4. Baumhueter S, et al. 1994. Blood. 84:2554.
- Gene ID
- 12490 View all products for this Gene ID
- View information about CD34 on UniProt.org
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