Alexa Fluor® 594 anti-human Granzyme A Antibody

Pricing & Availability
Clone
CB9 (See other available formats)
Other Names
Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Isotype
Mouse IgG1, κ
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Product Citations
publications
CB9_A594_Granzyme-A_Antibody_1_ICC_051019
Human PBMCs were fixed with 2% PFA for 10 min, permeabilized with 0.5% Triton X-100 for 10 min, and blocked with 5% FBS plus 5% mouse serum for 30 min at room temperature (RT). Then, cells were stained with 5 µg/ml Granzyme A (clone CB9) Alexa Fluor® 594 (red) 30 min, followed by two washes and co-stained with 10 µg/ml CD8 (clone HIT8a) Alexa Fluor® 488 (yellow) and 10 µg/ml CD56 (clone HCD56) Alexa Fluor® 647 (green) for 15 min at RT. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • CB9_A594_Granzyme-A_Antibody_1_ICC_051019
    Human PBMCs were fixed with 2% PFA for 10 min, permeabilized with 0.5% Triton X-100 for 10 min, and blocked with 5% FBS plus 5% mouse serum for 30 min at room temperature (RT). Then, cells were stained with 5 µg/ml Granzyme A (clone CB9) Alexa Fluor® 594 (red) 30 min, followed by two washes and co-stained with 10 µg/ml CD8 (clone HIT8a) Alexa Fluor® 488 (yellow) and 10 µg/ml CD56 (clone HCD56) Alexa Fluor® 647 (green) for 15 min at RT. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • CB9_A594_Granzyme-A_Antibody_2_ICFC_051019
    Human peripheral blood mononuclear cells were stained with CD8 APC and then intracellularly stained with Granzyme A (clone CB9) Alexa Flour® 594 (left) or mouse IgG1, κ Alexa Flour®594 isotype control (right). The data was acquired by BD LSRFortessa™ cell analyzer equipped with Yellow-Green Laser (561 nm).
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
507218 100 µg $245
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Description

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human Granzyme A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Trimble L, et al. 1998. Blood 91:585.
  2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
  3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
  4. Chen H, et al. 2005. J. Immunol. 175:591.
RRID
AB_2563964 (BioLegend Cat. No. 507218)

Antigen Details

Structure
Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Bioactivity
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources
Cytotoxic T cells, NK cells
Cell Targets
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors
Assisted by perforin
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.

Gene ID
3001 View all products for this Gene ID
UniProt
View information about Granzyme A on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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