Alexa Fluor® 488 anti-human Granzyme A Antibody

Pricing & Availability
CB9 (See other available formats)
Other Names
Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellularly stained with CB9 Alexa Fluor® 488
  • CB9_Alx488_081406
    Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellularly stained with CB9 Alexa Fluor® 488
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
507212 100 tests $225
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Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Purified human Granzyme A
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Trimble L, et al. 1998. Blood 91:585.
  2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
  3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
  4. Chen H, et al. 2005. J. Immunol. 175:591.
Product Citations
  1. Buggert M, et al. 2014. PLoS Pathog. 10:1004251. PubMed
  2. Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed
AB_528909 (BioLegend Cat. No. 507212)

Antigen Details

Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Assisted by perforin
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources
Cytotoxic T cells, NK cells
Cell Targets
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Assisted by perforin
Cell Type
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.

Gene ID
3001 View all products for this Gene ID
View information about Granzyme A on

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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