Pacific Blue™ anti-human Granzyme A Antibody

Pricing & Availability
Clone
CB9 (See other available formats)
Other Names
Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CB9_Blue_071108.jpg
Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
  • CB9_Blue_071108.jpg
    Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
507208 25 µg $95
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507207 100 µg $225
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Description

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human Granzyme A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Trimble L, et al. 1998. Blood 91:585.
  2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
  3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
  4. Chen H, et al. 2005. J. Immunol. 175:591.
Product Citations
  1. Cellerai C, et al. 2010. J Virol. 84:3868. PubMed
  2. Sanchez-Ruiz Y, et al. 2011. PLoS One. 6:e27057. PubMed
  3. Yamamoto T, et al. 2012. J Virol. 86:5877. PubMed
  4. Dimova T, et al. 2015. Proc Natl Acad Sci U S A. 112:556. PubMed
  5. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
Publication Library
RRID
AB_492996 (BioLegend Cat. No. 507208)
AB_439755 (BioLegend Cat. No. 507207)

Antigen Details

Structure
Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Bioactivity
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources
Cytotoxic T cells, NK cells
Cell Targets
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors
Assisted by perforin
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.

Gene ID
3001 View all products for this Gene ID
UniProt
View information about Granzyme A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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