Alexa Fluor® 488 anti-mouse FOXP3 Antibody

Pricing & Availability
Clone
MF-14 (See other available formats)
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Rat IgG2b, κ
Ave. Rating
1 reviews
Product Citations
publications
MF-14_AF488_FOXP3_Antibody_ICFC_1_042015
C57BL/6 splenocytes were surface stained with CD4 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone MF-14) Alexa Fluor® 488 (top) or rat IgG2b, κ Alexa Fluor® 488 isotype control (bottom).
  • MF-14_AF488_FOXP3_Antibody_ICFC_1_042015
    C57BL/6 splenocytes were surface stained with CD4 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone MF-14) Alexa Fluor® 488 (top) or rat IgG2b, κ Alexa Fluor® 488 isotype control (bottom).
  • MF-14_AF488_FOXP3_Antibody_ICFC_2_042015
  • MF-14_AF488_FOXP3_Antibody_ICFC_3_042015
    OCT frozen mouse lymph node 5 µm sections were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Slides were stained with Purified anti-mouse CD4 (clone RM4-5), followed by goat anti-rat IgG DyLight™ 594 (red). Slides were then stained with Alexa Fluor® 488 anti-mouse FOXP3 (clone MF-14) (green) and counterstained with DAPI (blue). Slides were mounted with Prolong Gold and imaged the next day.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
126405 25 µg $160
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126406 100 µg $315
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Description

FOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Ono M, et al. 2007. Nature 446:685.
  2. Hori S, et al. 2003. Science 299:1057.
  3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
  4. Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
  5. Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
  6. Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
Product Citations
  1. Fallarino F, et al. 2009. J Immunol. 183:6303. PubMed
  2. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  3. Horikoshi M, et al. 2012. PLoS One. 7:e51215. PubMed
  4. Fazio F, et al. 2014. Neuropharmacology. 81:237. PubMed
  5. Boyoglu-Barnum S, et al. 2014. J Virol. 88:10569. PubMed
  6. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  7. Crooks J, et al. 2017. Mediators Inflamm. 10.1155/2017/1380615. PubMed
  8. Imbratta C, et al. 2019. Sci Rep. 9:6135. PubMed
  9. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  10. Škuljec J, et al. 2017. Front Immunol. 1.114583333. PubMed
RRID
AB_1089114 (BioLegend Cat. No. 126405)
AB_1089113 (BioLegend Cat. No. 126406)

Antigen Details

Structure
50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
Distribution

Nuclear; expressed in Treg cells.

Function
Master regulatory gene in Treg cell development, crucial for immune homeostasis.
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
4. Fallarino F, et al. 2009. J. Immunol. 183:6033.
5. Barber A, et al. 2009 J. Immunol. 183:6939.
6. Nakashima H, et al. 2010. J. Immunol. 184:4637.

Regulation
Present at high level in T reg cells. Induced by T cell activation.
Gene ID
20371 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Go To Top Version: 5    Revision Date: 04/21/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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