PE/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_PECy7_092409
C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
  • 1A8_PECy7_092409
    C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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127617 25 µg 80€
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127618 100 µg 243€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chan C, et al. 2012. Hypertension. 60:1207. PubMed
  2. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  3. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  4. Bryant J, et al. 2014. J Immunol. 192:6092. PubMed
  5. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  6. Furuya Y, et al. 2014. J Virol. 88:9166. PubMed
  7. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  8. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
  9. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  10. Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed
  11. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  12. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  13. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  14. Douglas T, et al. 2015. J Immunol. 195: 2365-2373. PubMed
  15. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  16. Lee J, et al. 2015. Nat Commun. 6: 8499. PubMed
  17. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  18. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  19. Sanfilippo A, et al. 2015. Infect Immun . 83: 2976-2983. PubMed
  20. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  21. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  22. Chen F, et al. 2016. J Immunol. 196: 4390 - 4399. PubMed
  23. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  24. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  25. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
  26. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  27. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
  28. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  29. Lu S, et al. 2020. JCI Insight. 5:00. PubMed
  30. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  31. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  32. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  33. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  34. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  35. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  36. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  37. Singh S, et al. 2021. Cell Host Microbe. 29(3):347-361.e12. PubMed
  38. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  39. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  40. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  41. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  42. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  43. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  44. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  45. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  46. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  47. Braza MS et al. 2018. Immunity. 49(5):819-828 . PubMed
  48. Kaufmann E et al. 2018. Cell. 172(1-2):176-190 . PubMed
  49. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  50. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  51. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  52. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  53. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  54. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  55. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  56. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  57. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  58. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  59. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  60. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  61. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  62. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  63. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  64. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  65. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  66. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  67. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  68. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  69. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  70. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  71. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  72. Ngamsri KC, et al. 2020. Front Immunol. 11:407. PubMed
  73. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  74. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  75. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  76. Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed
  77. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  78. Mercer HL, et al. 2020. PLoS Pathog. 16:e1008572. PubMed
  79. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  80. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  81. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
RRID
AB_1877262 (BioLegend Cat. No. 127617)
AB_1877261 (BioLegend Cat. No. 127618)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC, CyTOF®, WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G 1A8 FC, IHC-F, IHC
Biotin anti-mouse Ly-6G 1A8 FC, IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC, IHC-F
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC, IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC, IHC-F, SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC, IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC, Depletion, IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F
Go To Top Version: 3    Revision Date: 01/06/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse Ly-6G (Maxpar® Ready)

  • Brilliant Violet 650™ anti-mouse Ly-6G

  • Brilliant Violet 711™ anti-mouse Ly-6G

  • Brilliant Violet 605™ anti-mouse Ly-6G

  • Brilliant Violet 785™ anti-mouse Ly-6G

  • PE/Dazzle™ 594 anti-mouse Ly-6G

  • APC/Fire™ 750 anti-mouse Ly-6G

  • PerCP anti-mouse Ly-6G

  • TotalSeq™-A0015 anti-mouse Ly-6G

  • TotalSeq™-C0015 anti-mouse Ly-6G

  • TotalSeq™-B0015 anti-mouse Ly-6G

  • Spark Blue™ 550 anti-mouse Ly-6G

  • Spark NIR™ 685 anti-mouse Ly-6G

  • Spark YG™ 593 anti-mouse Ly-6G

  • PE/Cyanine5 anti-mouse Ly-6G

  • PE/Fire™ 810 anti-mouse Ly-6G Antibody

  • PE/Fire™ 640 anti-mouse Ly-6G

  • Purified anti-mouse Ly-6G

  • Biotin anti-mouse Ly-6G

  • FITC anti-mouse Ly-6G

  • PE anti-mouse Ly-6G

  • Alexa Fluor® 647 anti-mouse Ly-6G

  • Pacific Blue™ anti-mouse Ly-6G

  • APC anti-mouse Ly-6G

  • PerCP/Cyanine5.5 anti-mouse Ly-6G

  • PE/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 700 anti-mouse Ly-6G

  • APC/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 488 anti-mouse Ly-6G

  • Brilliant Violet 421™ anti-mouse Ly-6G

  • Brilliant Violet 570™ anti-mouse Ly-6G

  • Ultra-LEAF™ Purified anti-mouse Ly-6G

  • Brilliant Violet 510™ anti-mouse Ly-6G

  • Alexa Fluor® 594 anti-mouse Ly-6G

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