PE anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
BM8_PE_122007
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with BM8 PE
  • BM8_PE_122007
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with BM8 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
123109 25 µg 28€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123110 100 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Application References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Michaud J, et al. 2017. BMC Microbiology . 10.1186/s12866-017-1036-0. PubMed
  2. Wang C, et al. 2017. J Ethnopharmacol. 10.1016/j.jep.2017.06.014. PubMed
  3. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  4. Dong G, et al. 2017. Biochim Biophys Acta. 10.1016/j.bbadis.2017.08.005. PubMed
  5. Sundar I, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.09.157. PubMed
  6. Lumbroso D, et al. 2018. Front Immunol. 9:358. PubMed
  7. Lim D, et al. 2018. Front Physiol. 9:47. PubMed
  8. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  9. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  10. Perrotta C, et al. 2018. Front Immunol. 9:1186. PubMed
  11. Guo HX, et al. 2018. World J Gastroenterol. 24:1779. PubMed
  12. Rashid K, et al. 2018. Sci Rep. 8:9023. PubMed
  13. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  14. Karki R et al. 2018. Cell. 173(4):920-933 . PubMed
  15. Truax AD et al. 2018. Cell host & microbe. 24(3):364-378 . PubMed
  16. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  17. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  18. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  19. Simonneau M, et al. 2018. Oncotarget. 9:36457. PubMed
  20. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  21. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  22. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  23. Pelka K, et al. 2018. Immunity. 48:911. PubMed
  24. Das LM, et al. 2019. Autophagy. 15:813. PubMed
  25. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  26. Agelidis A, et al. 2019. Front Immunol. 10:500. PubMed
  27. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  28. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  29. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  30. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
  31. William M, et al. 2018. J Immunol. 200:4102. PubMed
  32. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
  33. Tan T, et al. 2019. Nat Commun. 10:3322. PubMed
  34. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  35. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  36. Song C, et al. 2019. Nat Commun. 10:3745. PubMed
  37. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  38. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  39. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  40. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  41. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  42. Sun T, et al. 2017. Mucosal Immunol. 0.954861111. PubMed
  43. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  44. Tamura R, et al. 2017. Inflamm Regen. 36:26:00. PubMed
  45. Pagan JD, et al. 2018. Cell. 172:564. PubMed
  46. Zhang J, et al. 2018. Oncoimmunology. 7:e1461301. PubMed
  47. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  48. Zhao Y, et al. 2019. Mucosal Immunol. 1.298611111. PubMed
  49. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  50. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  51. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  52. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  53. Wang S, et al. 2020. J Cell Mol Med. 24:722. PubMed
  54. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  55. Yu JM, et al. 2019. Nat Commun. 4.388888889. PubMed
  56. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  57. Han MS, et al. 2020. Proc Natl Acad Sci U S A. 117:2751. PubMed
  58. Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
  59. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  60. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  61. Kaur A, et al. 2019. Cancer Discov. 9:64. PubMed
  62. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  63. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  64. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  65. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  66. Cullis J, et al. 2017. Cancer Immunol Res. 5:182. PubMed
  67. Huang WC, et al. 2020. NPJ Vaccines. 5:23. PubMed
  68. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  69. Samir P, et al. 2019. Nature. 573:590. PubMed
  70. Wang Q, et al. 2019. FASEB Bioadv. 1:609. PubMed
  71. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  72. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
  73. Yang Y, et al. 2020. Cell Death Discov. 0.293055556. PubMed
  74. Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
  75. Min C, et al. 2020. Cell Death Dis. 0.847916667. PubMed
  76. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  77. Xu M, et al. 2020. Cell. 178(6):1478-1492.e20.. PubMed
  78. Sun X, et al. 2020. Cell Metabolism. 31(1):189-206.e8.. PubMed
  79. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  80. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  81. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  82. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  83. Wang Q, et al. 2020. Respir Res. 0.981944444. PubMed
  84. Liu Y, et al. 2019. Am J Physiol Cell Physiol. 317:C762. PubMed
  85. Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed
  86. Hasan Z, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 304:221. PubMed
  87. Glass A, et al. 2013. J Immunol. 190:4830. PubMed
  88. Pashover-Schallinger E, et al. 2012. FASEB J. 26:3891. PubMed
  89. Lee T, et al. 2014. Mol Biol Cell. 25:583. PubMed
  90. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  91. Xiang Y, et al. 2014. Proc Natl Acad Sci U S A. . PubMed
  92. Feng L, et al. 2014. Biochem Pharmacol. 89:109. PubMed
  93. Horwitz E, et al. 2014. Cancer Discovery. 4:730. PubMed
  94. Zhang S, et al. 2014. Eur J Pharmacol. 733:45. PubMed
  95. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  96. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  97. Yahuafai J, et al. 2014. J Control Release. 28:192. PubMed
  98. Hyatt L, et al. 2014. PLoS One. 9:109072. PubMed
  99. Zhang J, et al. 2014. J Immunol. 193:5149. PubMed
  100. Lieber S, et al. 2014. Mol Pharmacol. 87:162. PubMed
  101. Yin T, et al. 2015. J Immunol. 194:429. PubMed
  102. Tang E, et al. 2015. J Lipid Res. 56:358. PubMed
  103. Liu Z, et al. 2015. Biol Reprod. 92:63. PubMed
  104. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  105. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  106. Erin N, et al. 2015. Brain Behav Immun. 48: 174-185. PubMed
  107. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  108. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  109. Peng X, et al. 2015. J Immunol. 195: 2797-2805. PubMed
  110. Elbaz M, et al. 2015. Mol Oncol. 9: 906-919. PubMed
  111. Nasser MW, et al. 2015. Cancer Res . 75: 974-985. PubMed
  112. Oghumu S, et al. 2014. Immunology. 143: 109-119. PubMed
  113. Izquierdo-Useros N, et al. 2014. J Immunol . 192:4852-4858. PubMed
  114. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  115. Liu Y, et al. 2015. Sci Rep. 5:17215. PubMed
  116. Wang Y, et al. 2015. Sci Rep. 5:16973. PubMed
  117. O'Donnell C, et al. 2015. Br J Cancer. 114: 37 - 43. PubMed
  118. Mitsuhashi A, et al. 2015. Nat Commun. 6: 8792. PubMed
  119. Liu Z, et al. 2016. J Biol Chem. 291: 1441 - 1447. PubMed
  120. Yin T, et al. 2016. Sci Rep. 18:19534. PubMed
  121. Kumagai S, et al. 2016. Cardiovasc Res. 109: 272 - 282. PubMed
  122. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  123. Peterson T, et al. 2016. Proc Natl Acad Sci U S A. 113: 4470-4475. PubMed
  124. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  125. Ikezoe K, et al. 2016. Sci Rep. 6: 25781. PubMed
  126. Li T, et al. 2016. Cell Death Dis. 7:e2258. PubMed
  127. Peng J, et al. 2016. Nat Commun. 7:12029. PubMed
  128. Yu S, et al. 2016. J Immunol. 197: 256 - 265. PubMed
  129. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
  130. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  131. Tencati M, Tapping R 2016. Infect Immun . 84: 2904 - 2913. PubMed
  132. Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
  133. Chen W, et al. 2016. J Immunol. 197: 3554 - 3565. PubMed
  134. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  135. Karki R, et al. 2016. Nature. 10.1038/nature20597. PubMed
  136. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  137. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  138. Lin J, et al. 2017. Sci Rep. 7:41722. PubMed
  139. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  140. Wang L, et al. 2020. Front Cell Dev Biol. 0.745138889. PubMed
  141. Cheng XS, et al. 2020. J Cell Mol Med. 24:8391. PubMed
  142. Enam SF, et al. 2020. J Neuroinflammation. 0.845138889. PubMed
  143. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  144. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  145. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
  146. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  147. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  148. Yang H, et al. 2020. Arterioscler Thromb Vasc Biol. 40:e350. PubMed
  149. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  150. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  151. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  152. Kuai Y, et al. 2020. Theranostics. 5.099305556. PubMed
  153. Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
  154. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
  155. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  156. Reimer E, et al. 2020. J Cell Sci. 134:00:00. PubMed
  157. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  158. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  159. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  160. Li N, et al. 2021. eLife. 10:00. PubMed
  161. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  162. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  163. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
  164. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  165. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  166. Yuan Q, et al. 2021. Cell Reports. 34(5):108724. PubMed
  167. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  168. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  169. Penninger P, et al. 2021. STAR Protocols. 2(1):100352. PubMed
  170. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  171. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  172. Devi S, et al. 2021. Immunity. 54(6):1219-1230.e7. PubMed
  173. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  174. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
RRID
AB_893498 (BioLegend Cat. No. 123109)
AB_893486 (BioLegend Cat. No. 123110)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account