PE/Dazzle 594 anti-mouse Ly-6G Antibody anti-Ly-6G

Pricing & Availability

Clone: 1A8 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ
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Product Citations: publications

1A8_PEDZL_Ly-6G_Antibody_FC_092215 — C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram). Data shown was gated on myeloid cell population.

Compare all formats See PE/Dazzle™ 594 spectral data

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127647

25 µg

109€

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127648

100 µg

259€

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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
Spix B, et al. 2022. Nat Commun. 13:318. PubMed
Njock MS, et al. 2022. J Extracell Vesicles. 11:e12228. PubMed
Bell CR, et al. 2022. Nat Commun. 13:2063. PubMed
Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed

RRID

AB_2566318 (BioLegend Cat. No. 127647)
AB_2566319 (BioLegend Cat. No. 127648)

Antigen Details

Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644 View all products for this Gene ID
UniProt: View information about Ly-6G on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
Alexa Fluor® 594 anti-mouse Ly-6G	1A8	IHC-F
Purified anti-mouse Ly-6G	1A8	FC,IHC-F,IHC
Biotin anti-mouse Ly-6G	1A8	FC,IHC
FITC anti-mouse Ly-6G	1A8	FC
PE anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 647 anti-mouse Ly-6G	1A8	FC,IHC-F
Pacific Blue™ anti-mouse Ly-6G	1A8	FC
APC anti-mouse Ly-6G	1A8	FC
PerCP/Cyanine5.5 anti-mouse Ly-6G	1A8	FC
PE/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 700 anti-mouse Ly-6G	1A8	FC,IHC
APC/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 488 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G	1A8	FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G	1A8	FC
Ultra-LEAF™ Purified anti-mouse Ly-6G	1A8	FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G	1A8	FC
Purified anti-mouse Ly-6G (Maxpar® Ready)	1A8	FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 711™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 605™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 785™ anti-mouse Ly-6G	1A8	FC
PE/Dazzle™ 594 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 750 anti-mouse Ly-6G	1A8	FC
PerCP anti-mouse Ly-6G	1A8	FC
TotalSeq™-A0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-C0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-B0015 anti-mouse Ly-6G	1A8	PG
Spark Blue™ 550 anti-mouse Ly-6G	1A8	FC
Spark NIR™ 685 anti-mouse Ly-6G	1A8	FC
Spark YG™ 593 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
PE/Cyanine5 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
Spark UV™ 387 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 640 anti-mouse Ly-6G	1A8	FC
Spark YG™ 570 anti-mouse Ly-6G	1A8	IHC-F,FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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