Purified anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1-1A8_Pure_061708.jpg
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 1-1A8_Pure_061708.jpg
    C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 2-1A8_Purified_Ly-6G_Antibody_IHC_102518
    Fresh, frozen mouse spleen was stained with purified Ly6G clone 1A8 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
  • 3-1A8_Purified_Ly-6G_Antibody_IHC_022013
    Mouse uterine tissue fixed in 10% formalin, paraffin embedded, and sliced to 4 µm. After deparaffination and antigen retrieval, sample was stained using an automatic slide stainer. The anti-mouse Ly6G primary antibody was applied at 1:500 dilution in blocking buffer for 1 hr at RT and DAB was used for visualization.
Compare all formats
Cat # Size Price Quantity Check Availability Save
127601 50 µg 49€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
127602 500 µg 102€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Raccosta L, et al. 2013. J Exp Med. 210:1711. PubMed
  2. Andrade E, et al. 2013. J Immunol. 191:4759. PubMed
  3. Ng L, et al. 2011. J Invest Dermatol. 131:2058. PubMed
  4. McCartney-Francis N, et al. 2014. J Leukoc Biol. 96:917. PubMed
  5. Her Z, et al. 2015. EMBO Mol Med. 7:24. PubMed
  6. Ilyas G, et al. 2015. J Hepatol. Available online 29 August 2015. PubMed
  7. Yago T, et al. 2015. J Exp Med. 212: 1267 - 1281. PubMed
  8. Sacramento L, et al. 2015. Infect Immun . 83: 4604 - 4616. PubMed
  9. Stempel H, et al. 2016. J Biol Chem. 291: 9762-9775. PubMed
  10. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  11. Lalazar G, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G377 - G386. PubMed
  12. Bennewitz MF, et al. 2020. Blood Adv. 0.351388889. PubMed
  13. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  14. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  15. Tilburg J, et al. 2020. J Thromb Haemost. 1.940277778. PubMed
  16. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  17. Louwe MC, et al. 2020. JACC Basic Transl Sci. 1.048611111. PubMed
  18. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  19. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  20. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  21. Watanabe K, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006513. PubMed
  22. Danobeitia J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183701. PubMed
  23. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  24. Bonnart C, et al. 2017. Parasite Immunol.. 10.1111/pim.12489. PubMed
  25. Maybin JA, et al. 2018. Nat Commun. 9:295. PubMed
  26. Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed
  27. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
  28. Qu S, et al. 2018. Int J Cancer. 8:6685. PubMed
  29. Vallelian F, et al. 2018. Pharmacol Res Perspect. 15:5422. PubMed
  30. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  31. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  32. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  33. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  34. Wang B et al. 2018. Cell stem cell. 22(2):206-220 . PubMed
  35. Bence Daniel et al. 2018. Immunity. 49(4):615-626 . PubMed
  36. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  37. Otxoa–de–Amezaga A, et al. 2019. Acta Neuropathol. 137:321. PubMed
  38. Simon N, et al. 2019. JCI Insight. 4:. PubMed
  39. Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed
  40. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  41. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  42. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  43. Boeckel SRV, et al. 2019. Sci Rep. 9:7356. PubMed
  44. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  45. Bianchi–Frias D, et al. 2019. Mol Cancer Res. 17:321. PubMed
  46. Lin L, et al. 2018. J Invest Dermatol. 138:1032. PubMed
  47. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  48. Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed
  49. Mottahedin A, et al. 2017. J Leukoc Biol. 101:297. PubMed
  50. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  51. Ajona D, et al. 2017. Cancer Discov. 0.773611111. PubMed
  52. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  53. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  54. Goel S, et al. 2017. Nature. 548:471. PubMed
  55. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  56. Verma M, et al. 2018. Brain Behav Immun. 69:223. PubMed
  57. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  58. Schmid AS, et al. 2018. Rheumatology (Oxford). 57:748. PubMed
  59. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  60. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  61. Shen S, et al. 2018. Front Immunol. 1.06875. PubMed
  62. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  63. Gu H, et al. 2018. Aging (Albany NY). 1.525694444. PubMed
  64. Luo J, et al. 2019. Clin Cancer Res. 25:808. PubMed
  65. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  66. Bhatt K, et al. 2018. mSphere. 3:e00352. PubMed
  67. Zhang M, et al. 2018. Front Immunol. 2.285416667. PubMed
  68. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
  69. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  70. Kapellos TS, et al. 2019. FASEB J. 33:6154. PubMed
  71. Otxoa–de–Amezaga A, et al. 2019. Stroke. 50:1548. PubMed
  72. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  73. Carper MB, et al. 2019. Cell Rep. 29:1660. PubMed
  74. Wang Y, et al. 2019. Cell. 179:1144. PubMed
  75. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  76. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  77. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  78. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  79. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  80. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  81. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  82. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  83. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  84. Paharik AE, et al. 2017. Cell Host Microbe. 22:746. PubMed
  85. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  86. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  87. Deng M, et al. 2019. J Exp Med. 216:2838. PubMed
  88. Kapellos TS, et al. 2017. Mediators Inflamm. 2017:4315412. PubMed
  89. Peterson LW, et al. 2017. J Exp Med. 214:3171. PubMed
  90. McArthur S, et al. 2020. J Clin Invest. 130:1156. PubMed
  91. Jung H, et al. 2019. Autophagy. 15:1990. PubMed
  92. Rehm SRT, et al. 2019. Sci Rep. 9:9925. PubMed
  93. Yao W, et al. 2019. Nature. 568:410. PubMed
  94. Prows DR, et al. 2020. American Journal of Physiology-Heart and Circulatory Physiology. 317(2):H405-H414.. PubMed
  95. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  96. Choudhury SR, et al. 2020. Cell. 178(5):1205-1221.e17.. PubMed
  97. Kang SH, et al. 2018. Oncoimmunology. 8:e1515057. PubMed
  98. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  99. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  100. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  101. Guiducci C, et al. 2010. J Exp Med. 207:2931. PubMed
  102. Sumagin R, et al. 2010. J Immunol. 185:7057. PubMed
  103. Reeves R 2010. J Immunol. 185:4904. PubMed
  104. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  105. Cain D, et al. 2011. PLoS One. 6:e19957. PubMed
RRID
AB_1089179 (BioLegend Cat. No. 127601)
AB_1089180 (BioLegend Cat. No. 127602)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC, CyTOF®, WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G 1A8 FC, IHC-F, IHC
Biotin anti-mouse Ly-6G 1A8 FC, IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC, IHC-F
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC, IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC, IHC-F, SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC, IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC, Depletion, IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F
Go To Top Version: 6    Revision Date: 10/25/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse Ly-6G (Maxpar® Ready)

  • Brilliant Violet 650™ anti-mouse Ly-6G

  • Brilliant Violet 711™ anti-mouse Ly-6G

  • Brilliant Violet 605™ anti-mouse Ly-6G

  • Brilliant Violet 785™ anti-mouse Ly-6G

  • PE/Dazzle™ 594 anti-mouse Ly-6G

  • APC/Fire™ 750 anti-mouse Ly-6G

  • PerCP anti-mouse Ly-6G

  • TotalSeq™-A0015 anti-mouse Ly-6G

  • TotalSeq™-C0015 anti-mouse Ly-6G

  • TotalSeq™-B0015 anti-mouse Ly-6G

  • Spark Blue™ 550 anti-mouse Ly-6G

  • Spark NIR™ 685 anti-mouse Ly-6G

  • Spark YG™ 593 anti-mouse Ly-6G

  • PE/Cyanine5 anti-mouse Ly-6G

  • PE/Fire™ 810 anti-mouse Ly-6G Antibody

  • PE/Fire™ 640 anti-mouse Ly-6G

  • Purified anti-mouse Ly-6G

  • Biotin anti-mouse Ly-6G

  • FITC anti-mouse Ly-6G

  • PE anti-mouse Ly-6G

  • Alexa Fluor® 647 anti-mouse Ly-6G

  • Pacific Blue™ anti-mouse Ly-6G

  • APC anti-mouse Ly-6G

  • PerCP/Cyanine5.5 anti-mouse Ly-6G

  • PE/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 700 anti-mouse Ly-6G

  • APC/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 488 anti-mouse Ly-6G

  • Brilliant Violet 421™ anti-mouse Ly-6G

  • Brilliant Violet 570™ anti-mouse Ly-6G

  • Ultra-LEAF™ Purified anti-mouse Ly-6G

  • Brilliant Violet 510™ anti-mouse Ly-6G

  • Alexa Fluor® 594 anti-mouse Ly-6G

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account