Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
MG1-45 (See other available formats)
Regulatory Status
Mouse (BALB/c) IgG1, κ
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Product Citations
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401407 100 µg £72
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401408 1 mg £102
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401411 5 mg £369
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401412 25 mg £964
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401413 50 mg £1611
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401414 100 mg £2314
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The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Trinitrophenol + KLH
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

FC, ICFC - Quality tested
WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozeki M, et al. 2008. J. Leukocyte Biol. 84:769. PubMed
  2. Kraetzel K, et al. 2008. Eur. Respair. J. 32:563. PubMed
  3. de Jong MA, et al. 2008. J Gen Virol. 89:2398. PubMed
  4. Romee R, et al. 2012 Blood. 120:4751. PubMed.
  5. Wong-Baeza I, et al. 2013. J. Immunol. 190:3216. PubMed.
Product Citations
  1. Gabriely G, et al. 2021. iScience. 24:103347. PubMed
  2. Chen F, et al. 2020. Cancer Med. 9:3584. PubMed
  3. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  4. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  5. Berg V, et al. 2020. Immunohorizons. 0.28125. PubMed
  6. White JM, et al. 2020. Mol Pharm. 1139:17. PubMed
  7. Vazquez Rodriguez G et al. 2018. Frontiers in immunology. 1.602083333 . PubMed
  8. Vazquez Rodriguez G, et al. 2017. Cancer Immunol Res. 10.1158/2326-6066.CIR-16-0150. PubMed
  9. Chou CC, et al. 2022. MAbs. 14:2029675. PubMed
  10. Gravastrand C, et al. 2017. Acta Biomaterialia. 10.1016/j.actbio.2017.05.052. PubMed
  11. Chen J, et al. 2021. J Invest Dermatol. 787:141. PubMed
  12. Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
  13. Gimpel AK, et al. 2022. Front Immunol. 12:761816. PubMed
  14. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
AB_11148722 (BioLegend Cat. No. 401407)
AB_11148942 (BioLegend Cat. No. 401408)

Antigen Details

Gene ID

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All Reagents
Description Clone Applications
Purified Mouse IgG1, κ Isotype Ctrl MG1-45 FC,ICFC,ICC,IHC,IP,WB,ChIP
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl MG1-45 FC,ICFC,WB,IP,ICC,IHC,FA
Go To Top Version: 2    Revision Date: 01/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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