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Purified Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG1-45 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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401401 50 µg £33
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401402 500 µg £89
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Description

The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ChIP - Verified
WB, IP, ICC, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
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  31. Fetz AE, et al. 2021. Acta Biomater. 130:281. PubMed
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  54. Huang Y, et al. 2021. Front Immunol. 12:649502. PubMed
  55. Kraetzel K, et al. 2008. Eur Respir J. 32:563. PubMed
  56. Shively M 2008. J Leukoc Biol. 84:769. PubMed
  57. Parashar D, et al. 2019. Cell Rep. 29:4389. PubMed
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RRID
AB_2801452 (BioLegend Cat. No. 401401)
AB_2801451 (BioLegend Cat. No. 401402)

Antigen Details

Gene ID
NA

Other Formats

View All Reagents
Description Clone Applications
Purified Mouse IgG1, κ Isotype Ctrl MG1-45 FC,ICFC,ICC,IHC,IP,WB,ChIP
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl MG1-45 FC,ICFC,WB,IP,ICC,IHC,FA
Go To Top Version: 3    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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