Ultra-LEAF™ Purified anti-human CD282 (TLR2) Antibody

Pricing & Availability
Clone
TL2.1 (See other available formats)
Workshop
HCDM listed
Other Names
Toll like receptor 2, TIL4, CD282
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
TL2_1
Human peripheral blood monocytes stained with TL2.1 FITC
  • TL2_1
    Human peripheral blood monocytes stained with TL2.1 FITC
Cat # Size Price Quantity Avail. Save
309717 100 µg £165
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309718 1 mg £437
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Description

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR2-transfected CHO cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, IHC-P, IHC-F, IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation. Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 309709). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 309716) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Flo T, et al. 2000. J. Immunol. 164:2064.
  2. Faure E, et al. 2001. J. Immunol. 166:2018.
  3. Sugawara S, et al. 2001. Infect. Immun. 69:4951.
  4. Droemann D, et al. 2003. Histochem. Cell Biol. 119:103.
  5. Chavakis E, et al. 2007. Circ. Res. 100:204.PubMed
  6. Fiala M, et al. 2007. Proc. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
  7. Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed
  8. Weiss DJ,et al. 2008.J. Leukoc. Biol. 83:48. PubMed
  9. Harris, KM., et al. 2011. J. Leukoc Biol. 90:727. PubMed.
  10. Elass-Rocahrd E, et al. 2012. J Biol Chem. 287:34432. PubMed.

Antigen Details

Structure
IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Distribution

Monocytes, granulocytes, endothelial and epithelial cells

Function
Activates innate host defense against bacterial pathogens
Ligand/Receptor
Microbial lipoproteins, peptidoglycans, lipoteichoic acid
Cell Type
Endothelial cells, Epithelial cells, Granulocytes, Monocytes, Neutrophils, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Lien E, et al. 1999. J. Biol. Chem. 274:33419.
2. Lien E, et al. 2001. J. Biol. Chem. 276:1873.
3. Sabroe I, et al. 2002. J. Immunol. 168:4701

Gene ID
7097 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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