Spark Violet™ 538 anti-human CD45, CD45, HI30

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: HI30
Workshop: IV N816
Other Names: LCA, T200
Isotype: Mouse IgG1, κ

HI30_SPKVL-538_CD45_ASR_Antibody_111921 — Typical results from human peripheral blood lymphocytes stained either with HI30 Spark Violet™ 538 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See Spark Violet™ 538 spectral data

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Size

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982320

500 µL

£312

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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Formulation

Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.

Concentration

400 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Suggested for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
Do not use this reagent beyond the expiration date stated on the label.
Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
Avoid prolonged exposure of the reagent or stained cells to light.
Spark Violet™ 538 conjugates may interact with biomolecules present in whole blood samples from certain donors that have been exposed to Polyethylene glycol (PEG) or similar molecules. The interpretation of the staining results must be evaluated by a qualified professional using the appropriate controls.

Antigen Details

Antigen References

Thomas ML. 1989. Annu Rev Immunol. 7:339.
Trowbridge IS, et al. 1994. Annu Rev Immunol.12:85.

Documentation

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/06/2021

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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