Analyte Specific Reagent. Analytical and performance characteristics are not established.
- Clone
- HI30
- Workshop
- IV N816
- Other Names
- LCA, T200
- Isotype
- Mouse IgG1, κ

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Typical results from human peripheral blood lymphocytes stained either with HI30 Spark Violet™ 538 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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982320 | 500 µL | £312 |
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Product DetailsProduct Details
- Reactivity
- Human
- Formulation
- Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
- Concentration
- 400 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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Suggested for Flow Cytometry
- Disclaimer
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WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
- Avoid prolonged exposure of the reagent or stained cells to light.
- Spark Violet™ 538 conjugates may interact with biomolecules present in whole blood samples from certain donors that have been exposed to Polyethylene glycol (PEG) or similar molecules. The interpretation of the staining results must be evaluated by a qualified professional using the appropriate controls.
Antigen Details
- Antigen References
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- Thomas ML. 1989. Annu Rev Immunol. 7:339.
- Trowbridge IS, et al. 1994. Annu Rev Immunol.12:85.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45
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Biotin anti-human CD45
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FITC anti-human CD45
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PE anti-human CD45
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PE/Cyanine5 anti-human CD45
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Purified anti-human CD45
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APC/Cyanine7 anti-human CD45
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PE/Cyanine7 anti-human CD45
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Alexa Fluor® 488 anti-human CD45
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Alexa Fluor® 647 anti-human CD45
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Pacific Blue™ anti-human CD45
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Alexa Fluor® 700 anti-human CD45
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PerCP anti-human CD45
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PerCP/Cyanine5.5 anti-human CD45
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Brilliant Violet 421™ anti-human CD45
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Brilliant Violet 570™ anti-human CD45
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Brilliant Violet 510™ anti-human CD45
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Brilliant Violet 605™ anti-human CD45
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Brilliant Violet 650™ anti-human CD45
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Purified anti-human CD45 (Maxpar® Ready)
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Brilliant Violet 785™ anti-human CD45
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Brilliant Violet 711™ anti-human CD45
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PE/Dazzle™ 594 anti-human CD45
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Alexa Fluor® 594 anti-human CD45
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APC/Fire™ 750 anti-human CD45
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Pacific Blue™ anti-human CD45
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APC anti-human CD45
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PE/Cyanine7 anti-human CD45
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PE/Dazzle™ 594 anti-human CD45
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TotalSeq™-A0391 anti-human CD45
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TotalSeq™-B0391 anti-human CD45
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TotalSeq™-C0391 anti-human CD45
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PerCP/Cyanine5.5 anti-human CD45
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APC/Fire™ 750 anti-human CD45
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PE/Fire™ 640 anti-human CD45
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APC/Fire™ 810 anti-human CD45
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Spark YG™ 570 anti-human CD45
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PE/Fire™ 700 anti-human CD45
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FITC anti-human CD45
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PerCP anti-human CD45
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Alexa Fluor® 660 anti-human CD45 Antibody
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Spark Violet™ 538 anti-human CD45
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Spark YG™ 593 anti-human CD45
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GMP APC/Fire™ 750 anti-human CD45
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Spark Violet™ 538 anti-human CD45
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GMP APC anti-human CD45
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Spark UV™ 387 anti-human CD45
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GMP Pacific Blue™ anti-human CD45
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GMP PerCP anti-human CD45
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GMP FITC anti-human CD45
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PE anti-human CD45
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GMP PE/Dazzle™ 594 anti-human CD45
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GMP PerCP/Cyanine5.5 anti-human CD45
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Spark Blue™ 515 anti-human CD45
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TotalSeq™-D0391 anti-human CD45
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