Recombinant Human VEGF-B167 (carrier-free)

Pricing & Availability
Other Names
Vascular endothelial growth factor B, VRF, VEGF-B, VEGF-B167
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Human_VEGF-B167_RECOM_CF_052119.png
When human VEGF-B167 is immobilized at 0.5 µg/mL, human Neuropilin-1 (Cat. No. 768002) binds with an EC50 of 0.1 – 0.4 µg/mL in a functional ELISA.
  • Human_VEGF-B167_RECOM_CF_052119.png
    When human VEGF-B167 is immobilized at 0.5 µg/mL, human Neuropilin-1 (Cat. No. 768002) binds with an EC50 of 0.1 – 0.4 µg/mL in a functional ELISA.
  • Human_VEGF-B167_RECOM_CF_2_052119.png
    Stability testing for Human VEGF-B167. Human VEGF-B167 was aliquoted in 35% Acetonitrile, 0.1% TFA at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with human Neuropilin-1.
Cat # Size Price Quantity Avail. Save
783902 10 µg £84
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783904 25 µg £144
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783906 100 µg £433
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Description

Vascular endothelial growth factor B (VEGF-B) is a member of the VEGF family of growth factors that are important regulators of angiogenesis, vasculogenesis, and lymphangiogenesis.  VEGF-B is a disulfide-linked dimeric protein that exists as two isoforms, VEGF-B167 and VEGF-B186, by alternate splicing.  VEGF-B167 binds strongly to heparan sulfate in the pericellular matrix due to a highly basic cysteine-rich heparin binding carboxy terminus, whereas VEGF-B186 is a secreted soluble form.  Both isoforms are expressed in most tissues concomitantly and bind to VEGF-R1 as well as to NRP-1, but they do not bind VEGF-R2 and VEGF-R3.  VEGF-B deficient mice are viable without any phenotype indicating that VEGF-B is redundant in angiogenesis in developing embryos and healthy adults.  The ability of VEGF-B to induce angiogenesis in most tissues is weak and maybe restricted to the ischemic heart.  VEGF-B has been detected in a wide range of tumours, but its’ role in tumour growth remains controversial.  VEGF-B is also expressed in the central nervous system and may have a role in neuroprotection.  VEGF-B deficient mice showed impaired recovery from cerebral ischemic injury and administration of VEGF-B stimulated neurogenesis in adult mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human VEGF-B167, amino acids Pro22-Arg188 (Accession # P49765) was expressed in E.coli.
Molecular Mass
The 167 amino acid recombinant protein has a predicted molecular mass of approximately 19 kD. The DTT-reduced protein migrates at approximately 24 kD and non-reduced protein migrate at approximately 48 kD by SDS-PAGE. The predicted N-terminal amino acid is Pro.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 35% Acetonitrile, 0.1% TFA
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When human VEGF-B167 is immobilized at 0.5 µg/mL, human Neuropilin-1 (Cat. No. 768002) binds with an EC50 of 0.1 – 0.4 µg/mL in a functional ELISA.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Expressed in most tissues including CNS

Function
Angiogenesis, neurogenesis
Interaction
Endothelial cells, CNS neurons
Ligand/Receptor
VEGF-R1, NRP-1 (CD304)
Bioactivity
Measured by its ability to bind human NRP-1
Cell Type
Endothelial cells, Neural Stem Cells, Mesenchymal Stem Cells, Embryonic Stem Cells
Biology Area
Angiogenesis, Cardiovascular Biology, Neuroscience, Stem Cells
Molecular Family
Growth Factors, Neurotrophic Factors
Antigen References
  1. Olofsson B, et al. 1996. J Biol Chem. 271:19310.
  2. Olofsson B, et al. 1998. Proc Natl Acad Sci USA. 95:11709.
  3. Aase K, et al. 1999. Dev. Dyn. 215:12-25.
  4. Bellomo D, et al. 2000. Circ Res. 86:E29.
  5. Niki T, et al. 2000. Clin Cancer Res. 6:2431.
  6. Makinen T, et al. 1999. J Biol Chem. 274:21217.
  7. Gunningham S, et al. 2001. Cancer Res. 61:3206.
  8. Sun Y, et al. 2004. J Cereb Blood Flow Metab. 24:1146.
  9. Li X, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:1614.
  10. Fischer C, et al. 2008. Nat Rev Cancer. 8:942.
Gene ID
7423 View all products for this Gene ID
UniProt
View information about VEGF-B167 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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