Recombinant Human IL-23 (mammalian expressed, carrier-free)

Pricing & Availability
Other Names
p19, IL-23 Subunit Alpha, IL-23p19, IL-23-A. p40, Interleukin-12 Subunit Beta, Interleukin 12B, IL-12B, Interleukin 12 P40.
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Human_IL-23_Mam-Expr_CF_RECOM_1_100419.png
Recombinant Human IL-23 induces IL-17A production in mouse splenocytes in a dose-dependent manner. The The ED50 for this effect is 0.2 – 1.2 ng/mL.
  • Human_IL-23_Mam-Expr_CF_RECOM_1_100419.png
    Recombinant Human IL-23 induces IL-17A production in mouse splenocytes in a dose-dependent manner. The The ED50 for this effect is 0.2 – 1.2 ng/mL.
  • Human_IL-23_Mam-Expr_CF_RECOM_2_100419.png
    Stability Testing for Recombinant Human IL-23. Human IL-23 was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to induce IL-17A production in mouse splenocytes in a dose-dependent manner. The ED50 for this effect is 0.2 – 1.2 ng/mL.
Cat # Size Price Quantity Avail. Save
787902 10 µg £124
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787904 25 µg £286
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787906 100 µg £840
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Description

Interleukin 23 (IL-23) is a member of the IL-12 family of cytokines, and it is comprised of two subunits, p19 and p40. The p19/p40 heterodimer is stabilized by a disulfide bond. The p40 subunit is shared by IL-23 and IL-12 cytokines; p19 mRNA is expressed in endothelial cells and polarized T cells, while p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12RB1 and IL-23R. Therefore, IL-23 and IL-12 share a common chain IL-12RB1 that interacts with p40. The second chain, IL-23R, interacts with p19. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling molecules. JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5. IL-23 has been associated with autoimmune inflammatory diseases such as psoriasis, colitis, gastritis, and arthritis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human IL-23, amino acid Ile23-Ser328/Arg20-Pro189 [Accession # P29460 (p40) -Linker - Q9NPF7 (p19)] was expressed in CHO cells. The carboxy terminus contains a GGS-6His tag.
Molecular Mass
The 502 amino acid recombinant protein has a predicted molecular mass of approximately 55.6 kD. The DTT-reduced and non-reduced protein migrates at approximately 65 kD and 60 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ile.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS pH7.2.
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human IL-23 induces IL-17A production in mouse splenocytes in a dose-dependent manner. The EC50 for this effect is 0.2 – 1.2 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

IL-23 is highly expressed by activated dendritic cells, macrophages, and epidermal Langerhans cells.

Function
IL-23 is the key cytokine in Th17 cell differentiation, Th17 cells differentiated from naive T cells are maintained and expanded by IL-23. IL-23 production by DCs is regulated by prostaglandin E2.
Interaction
Memory T cells, inflammatory macrophages, NK cells, dendritic cells, monocytes, and keratinocytes.
Ligand/Receptor
IL-23 binds to L-12RB1 and IL-23R
Bioactivity
Human IL-23 induces the production of IL-17A in mouse splenocytes.
Cell Type
Dendritic cells, Macrophages, Mesenchymal Stem Cells, NK cells, T cells, Th17
Biology Area
Adaptive Immunity, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Oppmann B, et al. 2000. Immunity 13:715.
  2. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
  3. Piskin G, et al. 2006. J. Immunol. 176:1908.
  4. McGeachy MJ, et al. 2007. Nat. Immunol. 8:1390.
  5. Wilson NJ, et al. 2007. Nat. Immunol. 8:950.
  6. Bonifac B, et al. 2008. Immunol. Rev. 226:132.
  7. Chizzolini C, et al. 2008. Blood 112:3696.
  8. Buonocore S, et al. 2010. Nature 464:1371.
  9. Harden JL, et al. 2015. J Autoimmun. 64:66.
  10. Fotiadou C, et al. 2018. Psoriasis (Auckl) 8:1.
Gene ID
3593 View all products for this Gene ID 51561 View all products for this Gene ID
UniProt
View information about IL-23 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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