Recombinant Human TGF-β1 (carrier-free)

Pricing & Availability
Other Names
TGFB, DPD1, transforming growth factor, Transforming Growth Factor Beta 1, TGF-Beta-1
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Product Citations
publications
Human_TGF-B1_RECOM_CF_1_052119
TGF-β1 inhibits the proliferation of mouse HT-2 cells induced by recombinant mouse IL-4 (Cat. No. 574302). The ED50 for this effect is 0.05 – 0.025 ng/mL.
  • Human_TGF-B1_RECOM_CF_1_052119
    TGF-β1 inhibits the proliferation of mouse HT-2 cells induced by recombinant mouse IL-4 (Cat. No. 574302). The ED50 for this effect is 0.05 – 0.025 ng/mL.
  • Human_TGF-B1_RECOM_CF_2_052419_updated
    Stability Testing for Recombinant Human TGF-β1. Recombinant Human TGF-β1 was aliquoted in <30% Acetonitrile, 0.1% TFA (trifluoroacetic acid) at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to inhibit the proliferation of mouse HT-2 cells induced by recombinant mouse IL-4 (Cat. No. 574302). The ED50 for this effect is 0.05 – 0.025 ng/mL.
Cat # Size Price Quantity Avail. Save
781802 10 µg £128
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781804 25 µg £252
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Description

TGF-β1 is synthesized in cells as a 390-amino acid. Furin cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide (LAP), and the 25-kD C-terminal portion of the precursor constitutes the mature TGF-β1. TGF-β activators can release TGF-β from LAP. These activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and integrins avb6 and avb8. Mouse TGF-β converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORγt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORγt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2 in combination with IL-6, IL-21 or IL-23.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human TGF-β1, amino acid Ala279 –Ser390 (Accession # P01137) was expressed in CHO cells.
Molecular Mass
The 112 amino acid recombinant protein has a predicted molecular mass of approximately 12.7 kD. The DTT-reduced and non-reduced proteins migrate at approximately 14 kD and 28 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in <30% Acetonitrile, 0.1% TFA (trifluoroacetic acid).
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Human TGF-β1 inhibits the proliferation of mouse HT-2 cells induced by recombinant mouse IL-4 (Cat. No. 574302) The ED50 for this effect is 0.05 – 0.25 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

TGF-β1 is secreted by numerous cells.

Function
TGF-β1 is a multifunctional cytokine that plays pivotal roles in diverse biological processes, including the regulation of cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, and tissue remodeling and repair. TGF-β1 is essential for wound healing, stimulates matrix molecule deposition and angiogenesis, and is an essential mediator of the pathologic scarring in fibrotic disorders.
Interaction
TGF-beta binding protein (LTBP), epithelial cells, fibroblasts, T cells, B cells, macrophages, and multiple cells respond to TGF-β1.
Ligand/Receptor
TGF-β1 binds to type II and type I serine/threonine kinase receptors, which initiate intracellular signals through activation of Smad proteins.
Cell Type
Embryonic Stem Cells, Endothelial cells, Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells, Osteoclasts, Th17, Tregs
Biology Area
Cardiovascular Biology, Immuno-Oncology, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Sun X, et al. 2013. J Virol. 87:10126.
  2. Benlhabib H, et al. 2015. J Biol Chem. 290:22409-22422.
  3. Lin C, et al. 2016. J Exp Med. 213:251-271.
  4. Chen P, et al. 2016. Sci Rep. 6:33407.
  5. de la Mare JA, et al. 2017. BMC Cancer. 17:202.
  6. Du C, et al. 2017. Int J Biochem Cell Biol. 90:17-28.
  7. Nüchel J, et al. 2018. Autophagy. 14:465.
Gene ID
7040 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 05/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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