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- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor 19, FGF-19, FGF19
- Ave. Rating
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- Product Citations
- publications
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Immobilized recombinant human FGFR4- Fc (Cat. No. 752504) is immobilized at 5 µg/mL, recombinant human FGF-19 binds in a dose-dependent manner in the presence of human FGF-19 biotinylated antibody. The ED50 range for this effect is 3 - 21 ng/mL. Human FGF-19 biotinylated antibody and HRP Avidin (Cat. No. 405103) were used to detect the binding. -
Stability testing for Recombinant Human FGF-19. Recombinant human FGF-19 was aliquoted in 1x PBS, 10% glycerol, and 5% trehalose at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were evaluated in a binding assay with immobilized recombinant human FGFR4- Fc (Cat. No. 752504) at 5 µg/mL and different concentration of human FGF-19 that binds in a dose-dependent manner in the presence of human FGF-19 biotinylated antibody. The ED50 range for this effect is 3 - 21 ng/mL. Human FGF-19 biotinylated antibody and HRP Avidin (Cat. No. 405103) were used to detect the binding.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 566004 | 25 µg | £161 | ||||
| 566006 | 100 µg | £421 | ||||
FGF-19 belongs to the fibroblast growth factor (FGF) family which includes FGF-19, FGF-21 and FGF-23. FGF-19 binds to FGFR with different affinity, especially with high affinity to FGFR4. α-Klotho and β-Klotho serve as co-receptors to facilitate the binding of FGF-19 to FGFRs in a tissue-specific manner. Though the members of FGF-19 subfamily do not bind to heparin with high affinity, heparin, together with β-Klotho, enhances the interaction between FGF-19 and FGFR4. FGF-19 is involved in regulation of several metabolic processes. Expression of FGF-19 and FGFR4 has been reported to be elevated in human hepatocellular carcinoma (HCC). FGF-19 overexpression in mice FGF-19/FGFR4 stimulates hepatocyte proliferation or tumor formation and invasion via FGF-19/FGFR4 interaction. IL-1β can inhibit β-Klotho expression and FGF-19-induced hepatocyte proliferation. FGF-19/FGFR4 also promotes ovarian cancer proliferation and invasion via AKT-MAPK pathway. FGF-19 are overexpressed in colon adenocarcinoma and lung squamous cell carcinoma. The antibody against FGF-19 can inhibit growth of colon tumor xenografts and hepatocellular carcinoma. FGF-19 is able to increase fatty acid oxidation by enhancing expression of Leptin receptor and by reducing expression of ACC2 [acetyl coenzyme A (CoA) carboxylase 2], the rate-limiting enzyme for fatty acid entry into the mitochondria. A bile acid-activated transcriptional factor, the farnesoid X receptor (FXR), positively regulates FGF-19 expression. In turn, FGF-19 controls bile acid biosynthesis by suppressing expression of a rate-limiting enzyme, cholesterol 7 α-hydroxylase (CYP7A1), via JNK-dependent pathway in human hepatocytes and mouse liver. FGF-19 increase glucose uptake in adipocytes in a β-Klotho-dependent manner.
Product DetailsProduct Details
- Source
- Human FGF-19, amino acid (Leu25-Lys216) (Accession: # O95750.1), was expressed in E.coli.
- Molecular Mass
- The 192 amino acid recombinant protein has a predicted molecular mass of approximately 21.43 kD. The DTT-reduced and non-reduced protein migrates at approximately 27 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, 10% glycerol, 5% trehalose
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human FGFR4 (Cat. No. 752504) is immobilized at 5 µg/mL, recombinant human FGF-19 binds in a dose-dependent manner. The ED50 range for this effect is 3 - 21 ng/mL. Human FGF-19 biotinylated antibody and HRP Avidin (Cat. No. 405103) were used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Fetal brain, cartilage, retina, adult gall bladder, hepatocellular carcinoma, ovarian cancer colon cancer, lung squamous cell carcinoma; Secreted
- Function
- FGF-19 regulates several metabolic processes, including bile acid biosynthesis, glucose uptake and fatty acid oxidation. FGF-19 also plays a role on tumor growth
- Interaction
- α-Klotho, β-Klotho
- Ligand/Receptor
- FGFR1, FGFR2, FGFR3, FGFR4
- Bioactivity
- Measured by its ability to bind human FGFR4
- Cell Type
- Epithelial cells, Granulocytes
- Biology Area
- Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Growth Factors
- Antigen References
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- Xie MH, et al. 1999. Cytokine. 11:729.
- Zhang X, et al. 2006. J Biol Chem. 281:15694-700.
- Wu X, et al. 2007. J Biol Chem. 282:29069-72.
- Wu X, et al. 2010. J Biol Chem. 285:5165-70.
- Miura S, et al. 2012. BMC Cancer. 12:56.
- Zhao Y, et al. 2016. Am J Physiol Endocrinol Meta. 310:E289-300.
- Hu L, et al. 2015. Oncol Rep. 34:2683-91.
- Wu X, et al. 2008. J Biol Chem. 283:33304-9.
- Holt JA, et al. 2003. Genes Dev. 17:1581-91.
- Song KH, et al. 2009. Hepatology. 49:297-305.
- Fu L, et al. 2004. Endocrinology. 145:2594-603.
- Kurosu H, et al. 2007. J Biol Chem. 282: 26687.
- Tomlinson E, et al. 2002. Endocrinology. 143:26687-26695.
- Desnoyers LR, et al. 2008. Oncogene. 27:85-97.
- Gene ID
- 9965 View all products for this Gene ID
- UniProt
- View information about FGF-19 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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