- UC7-13D5 (See other available formats)
- Other Names
- TCR-γ/δ, γ/δ TCR
- Armenian Hamster IgG
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- Product Citations
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T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, Stim, Depletion - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4. Although several papers have reported in vivo depletion3, 5,6, another study found that the effect of the antibody could be the functional blocking of the TCR signaling, rather than cell depletion7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 107515 - 107520).
Though clone UC7-13D5 has been used for the depletion of TCR γ/δ T cells in vivo3-5, there is a report suggesting that binding of clone UC7-13D5 to the γ/δ TCR leads to downregulation of the γ/δ TCR, making the γ/δ T cells undetectable although they are still present7.
(PubMed link indicates BioLegend citation)
- Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim)
- Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim)
- Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete)
- Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim)
- van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
- Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed
- Koenecke C, et al. 2009. Eur J Immunol. 39(2):372-9.
- Product Citations
AB_313311 (BioLegend Cat. No. 107502)
- Ig superfamily, associates with CD3 complex
T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
- Antigen recognition
- Some bacterial or tumor antigens bound MHC class I
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- Antigen References
1. Skarstein K, et al. 1995. Immunology 81:497.
2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167.
3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140.
4. Brandes M, et al. 2005. Science 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- View information about TCR gamma/delta on UniProt.org
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