Purified anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
UC7-13D5 (See other available formats)
Other Names
TCR-γ/δ, γ/δ TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
UC7-13D5_Pure_090808.jpg
C57BL/6 splenocytes stained with 17A2 (CD3) APC and purified UC7-13D5, followed by anti-Armenian hamster IgG PE
  • UC7-13D5_Pure_090808.jpg
    C57BL/6 splenocytes stained with 17A2 (CD3) APC and purified UC7-13D5, followed by anti-Armenian hamster IgG PE
Cat # Size Price Quantity Avail. Save
107502 500 µg £77
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Stim, Depletion - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4. Although several papers have reported in vivo depletion3, 5,6, another study found that the effect of the antibody could be the functional blocking of the TCR signaling, rather than cell depletion7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 107515 - 107520).

Though clone UC7-13D5 has been used for the depletion of TCR γ/δ T cells in vivo3-5, there is a report suggesting that binding of clone UC7-13D5 to the γ/δ TCR leads to downregulation of the γ/δ TCR, making the γ/δ T cells undetectable although they are still present7.

Application References

(PubMed link indicates BioLegend citation)
  1. Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim)
  3. Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete)
  4. Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim)
  5. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  6. Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed
  7. Koenecke C, et al. 2009. Eur J Immunol. 39(2):372-9.
Product Citations
  1. Pöllinger B, et al. 2011. J Immunol. 186:2602. PubMed
  2. Dong P, et al. 2014. PLoS One. 9:112964. PubMed
Publication Library
RRID
AB_313311 (BioLegend Cat. No. 107502)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex
Distribution

T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum

Function
Antigen recognition
Ligand/Receptor
Some bacterial or tumor antigens bound MHC class I
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Skarstein K, et al. 1995. Immunology 81:497.
2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167.
3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140.
4. Brandes M, et al. 2005. Science 309:264.

Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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