Purified anti-mouse IL-23 (p19) Antibody

Pricing & Availability
Clone
MMp19B2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 23 p19/p40, P19, IL-23A, IL23P19
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
IL-23_MU_ELISA_SingleReagent_082516
Immortalized murine bone marrow-derived macrophages were fixed with 2% paraformaldehyde, permeabilized with 0.1% Triton X100, stained with IL-23 (p19) (clone MMp19B2), followed by Alexa Fluor® 568 goat anti-mouse IgG. Image was visualized on an Olympus FV1000 confocal at 63x. Images provided by James Harris and Celia Peral de Castro, Trinity College.
  • IL-23_MU_ELISA_SingleReagent_082516
    Immortalized murine bone marrow-derived macrophages were fixed with 2% paraformaldehyde, permeabilized with 0.1% Triton X100, stained with IL-23 (p19) (clone MMp19B2), followed by Alexa Fluor® 568 goat anti-mouse IgG. Image was visualized on an Olympus FV1000 confocal at 63x. Images provided by James Harris and Celia Peral de Castro, Trinity College.
  • MMp19B2_IF_071712
    Immortalized murine bone marrow-derived macrophages were fixed with 2% paraformaldehyde, permeabilized with 0.1% Triton X100, stained with IL-23 (p19) (clone MMp19B2), followed by Alexa Fluor® 568 goat anti-mouse IgG. Image was visualized on an Olympus FV1000 confocal at 63x. Images provided by James Harris and Celia Peral de Castro, Trinity College.
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513801 50 µg £68.00
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513802 500 µg £205.00
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Description

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12.IL-23 is secreted by activated macrophages and dendritic cells.Human and mouse p19 share 70% aa sequence identity. The functional IL-23 receptor complex consists of two receptor subunits, a β1 subunit shared with the IL-12 receptor (IL-12R β1) and a β2-like subunit with STAT4 binding domain, termed the IL-23-specific receptor subunit (IL-23R).IL-23 has been shown to enhance IFN-γ production by memory T cells.Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the potent proinflammatory cytokine IL-17.IL-23 has been shown to be upregulated in certain autoimmune diseases and promote immunity in response to some viral and mycobacterial infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse p19 C-terminal peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ICC - Verified
Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 2.0 - 6.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-23 recombinant protein ranging from 1000 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified MMP19B2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed
Product Citations
  1. Ivanova DL, et al. 2019. J Immunol. 203:2944. PubMed
  2. Keswani T, Bhattacharyya A 2014. Exp Parasitol. 141:82. PubMed
RRID
AB_2233637 (BioLegend Cat. No. 513801)
AB_2280243 (BioLegend Cat. No. 513802)

Antigen Details

Structure
Member of the IL-12 cytokine family, heterodimeric cytokine composed of p40 subunit shared with IL-12 and a specific p19 subunit
Distribution

Secreted by activated dendritic cells and macrophages.

Function
Upregulated in viral infections, increased in psoriatic lesions and promotes immunity to mycobacteria.
Ligand/Receptor
IL-23 receptor (β1 subunit of IL-12 receptor and IL-23-specific subunit)
Bioactivity
Enhances IFN-γ secretion by memory (CD45RO) T cells and induces unique Th subset (designated Th-17) secreting IL-17.
Cell Type
Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Oppmann B, et al. 2000. Immunity 13:715.
2. Parham C, et al. 2002. J. Immunol. 168:5448.
3. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
4. Verreck FA, et al. 2004. Proc. Natl. Acad. Sci. USA 101:4560.
5. Lee E, et al. 2004. J. Exp. Med. 199:125.

Gene ID
83430 View all products for this Gene ID
UniProt
View information about IL-23 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 12/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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