Ultra-LEAF™ Purified anti-mouse IL-23 (p19) Antibody

Pricing & Availability
Clone
MMp19B2 (See other available formats)
Other Names
Interleukin 23 p19/p40, P19, IL-23A, IL23P19
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
MMp19B2_LEAF_IL23_Blocking_051513
IL-23 mediated secretion of IL-17A is inhibited by anti IL-23 clone MMp19B2. The data was generated from PMA 2.5 ng/ml and IL-2 20 ng/ml activated splenocytes (Balb/c), also treated with 5 ng/ml of recombinant IL-23 for 72 hours.
  • MMp19B2_LEAF_IL23_Blocking_051513
    IL-23 mediated secretion of IL-17A is inhibited by anti IL-23 clone MMp19B2. The data was generated from PMA 2.5 ng/ml and IL-2 20 ng/ml activated splenocytes (Balb/c), also treated with 5 ng/ml of recombinant IL-23 for 72 hours.
Cat # Size Price Quantity Avail. Save
513807 100 µg £158
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513808 1 mg £437
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Description

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12.IL-23 is secreted by activated macrophages and dendritic cells.Human and mouse p19 share 70% aa sequence identity. The functional IL-23 receptor complex consists of two receptor subunits, a β1 subunit shared with the IL-12 receptor (IL-12R β1) and a β2-like subunit with STAT4 binding domain, termed the IL-23-specific receptor subunit (IL-23R).IL-23 has been shown to enhance IFN-γ production by memory T cells.Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the potent proinflammatory cytokine IL-17.IL-23 has been shown to be upregulated in certain autoimmune diseases and promote immunity in response to some viral and mycobacterial infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse p19 C-terminal peptide
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of mouse IL-23 (5 ng/ml) in mouse splenocytes.

Application Notes

ELISA Capture: The purified MMP19B2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed

Antigen Details

Structure
Member of the IL-12 cytokine family, heterodimeric cytokine composed of p40 subunit shared with IL-12 and a specific p19 subunit
Distribution

Secreted by activated dendritic cells and macrophages.

Function
Upregulated in viral infections, increased in psoriatic lesions and promotes immunity to mycobacteria.
Ligand Receptor
IL-23 receptor (β1 subunit of IL-12 receptor and IL-23-specific subunit)
Bioactivity
Enhances IFN-γ secretion by memory (CD45RO) T cells and induces unique Th subset (designated Th-17) secreting IL-17.
Cell Type
Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Oppmann B, et al. 2000. Immunity 13:715.
2. Parham C, et al. 2002. J. Immunol. 168:5448.
3. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
4. Verreck FA, et al. 2004. Proc. Natl. Acad. Sci. USA 101:4560.
5. Lee E, et al. 2004. J. Exp. Med. 199:125.

Gene ID
83430 View all products for this Gene ID
UniProt
View information about IL-23 p19 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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