- HM40-3 (See other available formats)
- Regulatory Status
- Other Names
- Bp50, TNFRSF5
- Armenian Hamster IgM
- Ave. Rating
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- Product Citations
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CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- (BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block, Costim, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102908).
(PubMed link indicates BioLegend citation)
- Kawano T, et al. 1997. Science 278:1626. (Block)
- Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
- Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
- Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
- Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
- Ridge JP, et al. 1998. Nature 393:474. (Costim)
- Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
AB_312944 (BioLegend Cat. No. 102901)
AB_312945 (BioLegend Cat. No. 102902)
- TNFR superfamily, 48 kD
B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
- Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
- CD40L (CD154)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
- Gene ID
- 21939 View all products for this Gene ID
- View information about CD40 on UniProt.org
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