Purified anti-mouse CD19 Maxpar Ready Antibody anti-CD19

Pricing & Availability

Clone: 6D5 (See other available formats)
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
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Product Citations: publications

6D5_Purified_CD19_CyTOF_112113 — Mouse splenocytes stained with 169Tm-anti-TCRβ (H57-597) and 149Sm-anti-CD19 (6D5). Data provided by DVS Sciences.

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115547

100 µg

£65

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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁷.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
Olive V, et al. 2013. Elife. 2:822. PubMed
Miyai T, et al. 2014. PNAS. 111:11780. PubMed

Product Citations

Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

RRID

AB_2562806 (BioLegend Cat. No. 115547)

Antigen Details

Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD19 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD19	6D5	FC
Biotin anti-mouse CD19	6D5	FC
FITC anti-mouse CD19	6D5	FC
PE anti-mouse CD19	6D5	FC
PE/Cyanine5 anti-mouse CD19	6D5	FC
Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19	6D5	FC
Alexa Fluor® 488 anti-mouse CD19	6D5	FC
Alexa Fluor® 647 anti-mouse CD19	6D5	FC,IHC-F
Pacific Blue™ anti-mouse CD19	6D5	FC
Alexa Fluor® 700 anti-mouse CD19	6D5	FC
APC/Cyanine7 anti-mouse CD19	6D5	FC
PerCP anti-mouse CD19	6D5	FC
PerCP/Cyanine5.5 anti-mouse CD19	6D5	FC
Alexa Fluor® 594 anti-mouse CD19	6D5	IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19	6D5	FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19	6D5	FC
Brilliant Violet 605™ anti-mouse CD19	6D5	FC
Brilliant Violet 650™ anti-mouse CD19	6D5	FC
Brilliant Violet 785™ anti-mouse CD19	6D5	FC
Brilliant Violet 510™ anti-mouse CD19	6D5	FC
Purified anti-mouse CD19 (Maxpar® Ready)	6D5	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19	6D5	FC
Brilliant Violet 711™ anti-mouse CD19	6D5	FC
APC/Fire™ 750 anti-mouse CD19	6D5	FC
TotalSeq™-A0093 anti-mouse CD19	6D5	PG
Brilliant Violet 750™ anti-mouse CD19	6D5	FC
TotalSeq™-B0093 anti-mouse CD19	6D5	PG
Spark Blue™ 550 anti-mouse CD19	6D5	FC
Spark NIR™ 685 anti-mouse CD19	6D5	FC
TotalSeq™-C0093 anti-mouse CD19	6D5	PG
Ultra-LEAF™ Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody	6D5	FC
Spark YG™ 581 anti-mouse CD19	6D5	FC
APC/Fire™ 810 anti-mouse CD19	6D5	FC
Spark YG™ 570 anti-mouse CD19	6D5	IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody	6D5	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 03/05/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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