APC/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
Submit a Review
Product Citations
publications
HK1-4_APCCyanine7_Ly-6C_Antibody_020119
C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • HK1-4_APCCyanine7_Ly-6C_Antibody_020119
    C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
128025 25 µg £89
Check Availability


Need larger quantities of this item?
Request Bulk Quote
128026 100 µg £213
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  2. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  3. He J, et al. 2022. Front Immunol. 12:760138. PubMed
  4. Ippagunta S, et al. 2016. Proc Natl Acad Sci U S A. 113: E6162 - E6171. PubMed
  5. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  6. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  7. Sun CP, et al. 2021. PLoS Pathog. 17:e1009758. PubMed
  8. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
  9. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
  10. Heng Y, et al. 2020. Glia. . PubMed
  11. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  12. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  13. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  14. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  15. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
  16. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  17. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  18. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  19. Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed
  20. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  21. Motoyama S, et al. 2020. Cells. :9. PubMed
  22. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  23. Walker FC, et al. 2021. PLoS Pathog. 17:e1009402. PubMed
  24. Movita D, et al. 2015. J Virol. 89:4809. PubMed
  25. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  26. Hou X, et al. 2022. Cell Death Dis. 13:100. PubMed
  27. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  28. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  29. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  30. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  31. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  32. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  33. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  34. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  35. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  36. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  37. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  38. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  39. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  40. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  41. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  42. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  43. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  44. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  45. Terauchi R, et al. 2021. PLoS One. 16:e0239108. PubMed
  46. Wang SK, et al. 2021. JCI Insight. 6: . PubMed
  47. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  48. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  49. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  50. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  51. Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
  52. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  53. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  54. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  55. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  56. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  57. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  58. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  59. Capuccini B, et al. 2016. Sci Rep. 6:39258. PubMed
  60. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  61. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  62. Mulens-Arias V, et al. 2022. Pharmaceutics. 14:. PubMed
  63. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  64. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  65. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  66. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
  67. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  68. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  69. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  70. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  71. van de Garde M, et al. 2016. PLoS One. 11:e0166094. PubMed
  72. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  73. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  74. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  75. Zhang X, et al. 2020. Glia. . PubMed
  76. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
RRID
AB_10643867 (BioLegend Cat. No. 128025)
AB_10640120 (BioLegend Cat. No. 128026)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account