Purified anti-human/mouse Granzyme B Recombinant

Pricing & Availability
Clone
QA18A28 (See other available formats)
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A28_PURE_Granzyme-B_Antibody_1_061919
Human peripheral blood mononuclear cells were fixed and permeabilized, and then stained with Granzyme B (clone QA18A28, left) Purified or Rat IgG1, κ Purified isotype control (right) followed by anti-rat IgG1 FITC and CD8 APC.
  • QA18A28_PURE_Granzyme-B_Antibody_1_061919
    Human peripheral blood mononuclear cells were fixed and permeabilized, and then stained with Granzyme B (clone QA18A28, left) Purified or Rat IgG1, κ Purified isotype control (right) followed by anti-rat IgG1 FITC and CD8 APC.
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396402 100 µg £171
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
Neutrophils, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
  2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
  3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
  4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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