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Brilliant Violet 421™ anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA18A28 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A28_BV421_Granzyme-B_Antibody_041019
Human peripheral blood mononuclear cells were stained with CD8-FITC, fixed and permeabilized, and then stained with Granzyme B (clone QA18A28, left) Brilliant Violet 421™ or Rat IgG1, κ Brilliant Violet 421™ isotype control (right).
  • QA18A28_BV421_Granzyme-B_Antibody_041019
    Human peripheral blood mononuclear cells were stained with CD8-FITC, fixed and permeabilized, and then stained with Granzyme B (clone QA18A28, left) Brilliant Violet 421™ or Rat IgG1, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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396413 25 tests £136
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396414 100 tests £294
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Product Citations
  1. Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed
  2. Toomer G, et al. 2022. Viruses. 14:. PubMed
  3. Duan H, et al. 2021. J Clin Invest. 131:. PubMed
  4. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  5. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  6. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  7. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  8. Wilson TL, et al. 2022. Cancer Discov. 12:2098. PubMed
  9. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
RRID
AB_2810602 (BioLegend Cat. No. 396413)
AB_2810603 (BioLegend Cat. No. 396414)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
Neutrophils, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
  2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
  3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
  4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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