- MHM-88 (See other available formats)
- Regulatory Status
- Other Names
- Immunoglobulin M
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Human Ig cocktail
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
- Product Citations
AB_2563776 (BioLegend Cat. No. 314527)
- Ig family
- B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
- Cell Type
- B cells
- Biology Area
- Gene ID
- 3507 View all products for this Gene ID
- View information about IgM on UniProt.org
Related Pages & Pathways
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other FormatsView All IgM Reagents Request Custom Conjugation
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human IgM
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Pacific Blue™ anti-human IgM
Brilliant Violet 421™ anti-human IgM
APC/Cyanine7 anti-human IgM
Brilliant Violet 570™ anti-human IgM
Brilliant Violet 510™ anti-human IgM
Brilliant Violet 605™ anti-human IgM
Brilliant Violet 650™ anti-human IgM
Purified anti-human IgM (Maxpar® Ready)
PE/Dazzle™ 594 anti-human IgM
PE/Cyanine7 anti-human IgM
Alexa Fluor® 488 anti-human IgM
Alexa Fluor® 647 anti-human IgM
Alexa Fluor® 700 anti-human IgM
Brilliant Violet 711™ anti-human IgM
TotalSeq™-A0136 anti-human IgM
TotalSeq™-C0136 anti-human IgM
Brilliant Violet 785™ anti-human IgM
APC/Fire™ 750 anti-human IgM
TotalSeq™-B0136 anti-human IgM
Spark Violet™ 423 anti-human IgM Antibody
TotalSeq™-D0136 anti-human IgM
Spark Blue™ 550 anti-human IgM