Loading...

Purified anti-human IgM (Maxpar® Ready) Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
MHM-88_Purified_IgM_Cytof_061014.jpg
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 172Yb-anti-IgM (MHM-88). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.
  • MHM-88_Purified_IgM_Cytof_061014.jpg
    Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 172Yb-anti-IgM (MHM-88). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.
Compare all formats
Cat # Size Price Quantity Check Availability Save
314527 100 µg £89
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  2. Gruber CN, et al. 2020. Cell. 183:982. PubMed
  3. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  4. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
RRID
AB_2563776 (BioLegend Cat. No. 314527)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 2    Revision Date: 07/22/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human IgM

  • PE anti-human IgM

  • Biotin anti-human IgM

  • FITC anti-human IgM

  • APC anti-human IgM

  • PerCP/Cyanine5.5 anti-human IgM

  • Pacific Blue™ anti-human IgM

  • Brilliant Violet 421™ anti-human IgM

  • APC/Cyanine7 anti-human IgM

  • Brilliant Violet 570™ anti-human IgM

  • Brilliant Violet 510™ anti-human IgM

  • Brilliant Violet 605™ anti-human IgM

  • Brilliant Violet 650™ anti-human IgM

  • Purified anti-human IgM (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human IgM

  • PE/Cyanine7 anti-human IgM

  • Alexa Fluor® 488 anti-human IgM

  • Alexa Fluor® 647 anti-human IgM

  • Alexa Fluor® 700 anti-human IgM

  • Brilliant Violet 711™ anti-human IgM

  • TotalSeq™-A0136 anti-human IgM

  • TotalSeq™-C0136 anti-human IgM

  • Brilliant Violet 785™ anti-human IgM

  • APC/Fire™ 750 anti-human IgM

  • TotalSeq™-B0136 anti-human IgM

  • Spark Violet™ 423 anti-human IgM Antibody

  • TotalSeq™-D0136 anti-human IgM

  • Spark Blue™ 550 anti-human IgM
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account