Purified anti-GRB2 Antibody

Pricing & Availability
Clone
5G3-E7-F11 (See other available formats)
Other Names
Growth Factor Receptor Bound Protein 2, ASH
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5G3-E7-F11_Purified_GRB2_Antibody_1_061819.png
Western blot of purified anti-GRB2 antibody (clone 5G3-E7-F11). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5G3-E7-F11_Purified_GRB2_Antibody_1_061819.png
    Western blot of purified anti-GRB2 antibody (clone 5G3-E7-F11). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5G3-E7-F11_Purified_GRB2_Antibody_2_061819.png
    IHC staining of purified anti-GRB2 antibody (clone 5G3-E7-F11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R,, the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 5G3-E7-F11_Purified_GRB2_Antibody_3_061819.png
    IHC staining of purified anti-GRB2 antibody (clone 5G3-E7-F11) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 5G3-E7-F11_Purified_GRB2_Antibody_4_061819.png
    IHC staining of purified anti-GRB2 antibody (clone 5G3-E7-F11) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
867101 25 µg £60
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867102 100 µg £158
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Description

Growth factor receptor bound protein 2 (GRB2) is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. GRB2 is ubiquitously expressed, and has been shown to be essential to various cellular functions. Due to its involvement in cell growth, cell proliferation and metabolism, GRB2 has been linked to multiple tumor malignancies, and thus is a potential therapeutic target for cancer therapy.  Recent neurodevelopmental studies also suggest that variations in intracellular levels of adapter proteins such as GRB2 may represent an underlying mechanism that links exogenous signals to cell fate determination.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human GRB2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 2 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
GRB2 is a 217 amino acid protein with predicted and observed molecular mass of ~25 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, nucleus, cytosol, endosome, and Golgi apparatus

Function
GRB2 is an adapter protein that binds to activated SH2-containing proteins and plays an important role in determining cell fate.
Interaction
EGF receptors, PDGF receptors, and other proteins containing SH2 or SH3 domains.
Cell Type
Microglia, Neurons
Biology Area
Cancer Biomarkers, Cell Death, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family
Adaptor Proteins
Antigen References
  1. Ijaz M, et al. 2018. Protein Pept Lett. 24(12):1084
  2. Paff M, et al. 2014. Hum Vaccin Immunother. 10(11):3322
  3. Belov AA and Mohammadi M. 2012. Sci Signal. 5(249):pe49
Gene ID
2885 View all products for this Gene ID 14784 View all products for this Gene ID 81504 View all products for this Gene ID
UniProt
View information about GRB2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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