Purified anti-Cyclin B1 Antibody

Pricing & Availability
Clone
V152 (See other available formats)
Regulatory Status
RUO
Other Names
CCNB1
Isotype
Mouse IgG1
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Product Citations
publications
V152_Pure_Cyclin-B1_Antibody_1_092418
Total cell lysates (15 µg total protein) from HEL, HeLa, A549, Jurkat, and NIH/3T3 cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Cyclin B1 Antibody, clone V152, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • V152_Pure_Cyclin-B1_Antibody_1_092418
    Total cell lysates (15 µg total protein) from HEL, HeLa, A549, Jurkat, and NIH/3T3 cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Cyclin B1 Antibody, clone V152, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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647901 25 µg £72.00
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647902 100 µg £154.00
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Description

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hamster Cyclin B1
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2.5 - 5.0 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.

Application References

(PubMed link indicates BioLegend citation)
  1. Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed.
  2. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed
  3. Matsumura F, et al. 2011. J. Biol Chem. PubMed
Product Citations
  1. Matsumura F, et al. 2011. J Biol Chem. 286:10825. PubMed
RRID
AB_2072274 (BioLegend Cat. No. 647901)
AB_2244210 (BioLegend Cat. No. 647902)

Antigen Details

Structure
Belongs to the cyclin family. Cyclin AB subfamily.
Distribution

Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.

Interaction
CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Morgan DO, et al.1995. Nature 374:131

Gene ID
891 View all products for this Gene ID
UniProt
View information about Cyclin B1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/21/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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