Purified anti-Ataxin-2 Antibody

Pricing & Availability
Clone
A17203C (See other available formats)
Other Names
Spinocerebellar ataxia type 2 protein, Trinucleotide repeat-containing gene 13 protein, ATX2, SCA2, TNRC13, SCA2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17203C_Purified_Ataxin-2_Antibody_1_062619.png
Western blot purified anti-Ataxin-2 antibody (clone A17203C). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate; Lane 4: 10 µg of SH-SY5Y cell lysate. The blots were incubated with 1 µg/mL of clone A17203C or mouse IgG2b overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17203C_Purified_Ataxin-2_Antibody_1_062619.png
    Western blot purified anti-Ataxin-2 antibody (clone A17203C). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate; Lane 4: 10 µg of SH-SY5Y cell lysate. The blots were incubated with 1 µg/mL of clone A17203C or mouse IgG2b overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17203C_Purified_Ataxin-2_Antibody_2_062619.png
    IHC staining of purified anti-Ataxin-2 antibody (clone A17203C) on formalin-fixed paraffin-embedded human spinal cord tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17203C_Purified_Ataxin-2_Antibody_3_062619.png
    IHC staining of purified anti-Ataxin-2 antibody (clone A17203C) on formalin-fixed paraffin-embedded mouse spinal cord tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17203C_Purified_Ataxin-2_Antibody_4_062619.png
    IHC staining of purified anti-Ataxin-2 antibody (clone A17203C) on formalin-fixed paraffin-embedded rat spinal cord tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
868001 25 µg £60
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868002 100 µg £158
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Description

Ataxin-2 is a polyglutamine protein involved in several biological processes including regulation of mRNA translation. Ataxin-2 contains 2 domains, Sm1 and Sm2, that are implicated in RNA splicing and protein interactions. In the brain, ataxin-2 is expressed in the hypothalamus, the brain region that is responsible for controlling energy balance and metabolism homeostasis. Mutations in ataxin-2 are associated with the development of spinocerebellar ataxia type 2.  Ataxin-2 mutant protein with intermediate-length polyglutamine expansion (27-33 polyQ) modifies TDP-43 toxicity and is associated with an increased risk of developing amyotrophic lateral sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ataxin-2 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human ataxin-2 is a 1,313 amino acid protein with a predicted molecular mass of 140 kD.
Distribution

Tissue distribution: Brain, spinal cord, heart, liver, skeletal muscle, pancreas, and placenta
Cellular distribution: Cytoplasm

Function
Ataxin-2 is involved in the RNA splicing, protein interactions, and metabolic homeostasis.
Interaction
Ataxin-2 interacts with TARDBP, RBFOX1, polyribosomes, SH3GL2, SH3GL3, SH3KBP1, CBL, EGFR, and ATXN2L.
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Synaptic Biology
Antigen References

Carmo-Silva S, et al. 2017. Trends Endocrinol Metab. 28(4):309-318.
van den Heuvel DM, et al. 2014. Trends Mol Med. 20(1):25-35.
Becker LA, et al. 2017. Nature. 544(7650):367-371.
Scoles DR, et al. 2017. Nature. 544(7650):362-366

Gene ID
6311 View all products for this Gene ID
UniProt
View information about Ataxin-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/26/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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