Purified anti-Reptin (RuvBL2) Antibody

Pricing & Availability
Clone
O91A6 (See other available formats)
Other Names
RuvB Like AAA ATPase 2, INO80 Complex Subunit J, 48 KDa TATA Box-Binding Protein-Interacting Protein, Repressing pontin 52, TIP49b, TIP60-associated protein 54-beta, 51 kD Erythrocyte Cytosolic Protein, RVB2
Isotype
Mouse IgG1
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Product Citations
publications
O91A6_PURE_Reptin_Antibody_1_101419.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Reptin (RuvBL2) Antibody (clone O91A6) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • O91A6_PURE_Reptin_Antibody_1_101419.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Reptin (RuvBL2) Antibody (clone O91A6) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • O91A6_PURE_Reptin_Antibody_2_101419.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1.0 µg/mL (1:500 dilution) of either Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102, panel A) or Purified anti-Reptin (RuvBL2) Antibody (clone O91A6, panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • O91A6_PURE_Reptin_Antibody_3_101419.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102) or Purified anti-Reptin (RuvBL2) Antibody (clone O91A6). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Reptin (RuvBL2) Antibody (clone O91A6). Proteins were visualized by chemiluminescence detection using HRP anti-mouse IgG, light chain specific Antibody. Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
933801 25 µg £57
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933802 100 µg £144
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Description

Reptin (RuvBL2) belongs to the AAA+ (ATPases associated with diverse cellular activities) superfamily of proteins and contains Walker A and B motifs, which modulate the binding and hydrolysis of ATP. Reptin (RuvBL2) plays a role in chromatin remodeling and transcriptional regulation, thereby promoting the growth and survival of cells. Chromatin remodeling complexes such as Tip60 and Ino80 require Reptin (RuvBL2) for the regulation of DNA damage responses and repair. In addition, Reptin (RuvBL2) interacts with oncogenic transcriptional factors such as c-Myc and β-catenin. Overexpression of Reptin (RuvBL2) is associated with the development and poor prognosis of hepatocellular carcinoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human Reptin (RuvBL2) protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes both human and mouse Reptin (RuvBL2).

Methanol permeabilization produced stronger signal than Triton X-100 permeabilization in ICC testing.

Antigen Details

Structure
Reptin (RuvBL2) is a 463 amino acid protein with a predicted molecular weight of 51 kD.
Distribution

Mainly localized in nucleus. Also present in cytoplasm/Ubiquitously expressed

Function
Chromatin remodeling, transcriptional regulation
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Izumi N, et al. 2010. Sci. Signal. 3:ra27.
  2. Huber O, et al. 2008. Cancer Research. 68:6873.
  3. Gallant P. 2007. Trends in cell biology. 17:187.
  4. Kim J, et al. 2006. Nat Cell Biol. 8:631-9.
     
Gene ID
10856 View all products for this Gene ID
UniProt
View information about Reptin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/14/2019

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