PerCP/Cyanine5.5 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
H57-597_PerCPCyanine55_TCR-B-chain_Antibody_110118
C57BL/6 splenocytes were stained with CD3e APC and TCR ß chain (clone H57-597) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • H57-597_PerCPCyanine55_TCR-B-chain_Antibody_110118
    C57BL/6 splenocytes were stained with CD3e APC and TCR ß chain (clone H57-597) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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109227 25 µg £77
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109228 100 µg £197
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Oh–Hora M, et al. 2019. J Immunol. 202:2616. PubMed
  2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  3. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  4. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  5. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  6. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  7. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  8. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  9. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  10. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  11. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  12. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
  13. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  14. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  15. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  16. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  17. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  18. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  19. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  20. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  21. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  22. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  23. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  24. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  25. Kunis G, et al. 2013. Brain. 136:3427. PubMed
  26. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  27. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  28. Tong Q, et al. 2022. FEBS Open Bio. 12:1542. PubMed
  29. Hutter K, et al. 2020. FEBS J. . PubMed
  30. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  31. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  32. Krollmann C, et al. 2022. STAR Protoc. 3:101309. PubMed
  33. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  34. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  35. Dodard G, et al. 2020. Cell Reports. 32(1):107855.. PubMed
  36. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
  37. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  38. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  39. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  40. Kim M, et al. 2018. Immunity. 49:151. PubMed
  41. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  42. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  43. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  44. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  45. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  46. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  47. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  48. Sun T, et al. 2017. J Cell Biol. 216:199. PubMed
  49. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  50. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  51. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  52. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  53. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  54. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  55. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
  56. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  57. Hester AK, et al. 2022. Cell Rep. 38:110363. PubMed
  58. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_1575176 (BioLegend Cat. No. 109227)
AB_1575173 (BioLegend Cat. No. 109228)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 3    Revision Date: 11/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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